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F2653

Sigma-Aldrich

Anti-Mouse IgG (Fab specific) F(ab′)2 fragment–FITC antibody produced in goat

flow cytometry grade, affinity isolated antibody, buffered aqueous solution

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MDL number:
NACRES:
NA.46

biological source

goat

Quality Level

conjugate

FITC conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

grade

flow cytometry grade

clone

polyclonal

form

buffered aqueous solution

technique(s)

flow cytometry: 1:100
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:160

storage temp.

−20°C

target post-translational modification

unmodified

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This Item
F8521F5387F8646
technique(s)

flow cytometry: 1:100, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:160

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:320

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100, indirect immunofluorescence: 1:32

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:320

conjugate

FITC conjugate

conjugate

FITC conjugate

conjugate

FITC conjugate

conjugate

FITC conjugate

biological source

goat

biological source

goat

biological source

goat

biological source

goat

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

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General description

IgG, a monomer, is the principal antibody circulating in blood which assist in phagocytic destruction of foreign microorganism and is the only class of Ig that can cross the placenta in humans. Cell-sorting done by differential binding of anti-mouse IgG (Fab Specific) - FITC antibody separates tumour cells from macrophages. This antibody can be used to incubate somatic cell nuclear transfer (SCNT) rat embryos for immunocytochemical observation. It also can be used as a secondary antibody to study the dynamics of activated B lymphocytes in small intestine, Peyer′s patch germinal center as well as non-germinal center, spleen and the mesenteric lymph node. Anti-mouse IgG antibody reacts specifically with all mouse immunoglobulins.
Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. An immunoglobulin has two heavy chains and two light chains connected by a disulfide bond. It mainly helps in immune defense. It is a glycoprotein. IgG is a major class of immunoglobulin. Mouse consists of five immunoglobulin classes- IgM, IgG, IgA, IgD and IgE. Mouse IgG is further divided into five classes- IgG1, IgG2a, IgG2b and IgG3.

Specificity

Binds all mouse Igs.
Useful when trying to avoid background staining due to the presence of Fc receptors.

Immunogen

Purified Fab fragments of mouse IgG.

Application

Anti-Mouse IgG (Fab Specific) FITC Conjugate antibody is used in traditional method of immunocytochemical assessment of ZAP-70 expression. It can also be used in immunocytochemical observation of the SCNT embryos.
Anti-Mouse IgG (Fab specific) F(ab′)2 fragment-FITC antibody produced in goat has been used in:
  • indirect immunofluorescence staining
  • immunofluorescence staining
  • flow cytometry

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Flow cytometry/Cell sorting (1 paper)

Biochem/physiol Actions

Immunoglobulin G (IgG) participates in hypersensitivity type II and type III reactions. IgG helps in opsonization, complement fixation and antibody dependent cell mediated cytotoxicity.

Other Notes

Antibody adsorbed with bovine, equine and human serum proteins.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Preparation Note

Adsorbed to reduce background staining with bovine, horse or human samples.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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Eabha O'Driscoll et al.
Journal of clinical medicine, 12(7) (2023-04-14)
Optic nerve head (ONH) cupping is a clinical feature of glaucoma associated with extracellular matrix (ECM) remodelling and lamina cribrosa (LC) fibrosis. Peripapillary atrophy (PPA) occurs commonly in glaucoma, and is characterised by the loss of retinal pigment epithelium (RPE)
Xiaobing He et al.
Stem cells (Dayton, Ohio), 27(7), 1487-1495 (2009-06-23)
Highly tumorigenic cancer cell (HTC) populations have been identified for a variety of solid tumors and assigned stem cell properties. Strategies for identifying HTCs in solid tumors have been primarily empirical rather than rational, particularly in epithelial tumors, which are
All 4 di-leucine motifs in the first hydrophobic domain of the E5 oncoprotein of human papillomavirus type 16 are essential for surface MHC class I downregulation activity and E5 endomembrane localization
Cortese MS, et al.
International Journal of Cancer. Journal International Du Cancer, 126(7), 1675-1682 (2010)
Agnieszka Bojarska-Junak et al.
Folia histochemica et cytobiologica, 43(1), 19-23 (2005-05-06)
The ZAP-70 protein is a member of the Syk/ZAP protein tyrosine kinase family, normally expressed in T cells and NK cells but not found in normal, mature B cells. The protein plays a critical role in the initiation of T-cell
Mustapha Irnaten et al.
Investigative ophthalmology & visual science, 59(2), 831-842 (2018-02-08)
Optic nerve cupping in glaucoma is characterized by remodeling of the extracellular matrix (ECM) and fibrosis in the lamina cribrosa (LC). We have previously shown that glaucoma LC cells express raised levels of ECM genes and have elevated intracellular calcium

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