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M6898

Sigma-Aldrich

Anti-Mouse IgG (Fab specific) antibody produced in goat

affinity isolated antibody, buffered aqueous solution

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Synonym(s):
Anti-Mouse Fab Antibody, Anti-Mouse Fab Antibody - Anti-Mouse IgG (Fab specific) antibody produced in goat
MDL number:

biological source

goat

Quality Level

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

technique(s)

indirect ELISA: 1:20,000

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

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This Item
M4155A9917A3682
biological source

goat

biological source

goat

biological source

goat

biological source

goat

technique(s)

indirect ELISA: 1:20,000

technique(s)

Ouchterlony double diffusion: suitable, indirect ELISA: 1:15,000

technique(s)

direct ELISA: 1:60,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:150, western blot: 1:80,000-1:160,000

technique(s)

direct ELISA: 1:40,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:150, western blot: 1:80,000-160,000 using detecting β-actin in total cell extract of HeLa cells (5-10 μg per lane)

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

form

buffered aqueous solution

form

-

form

buffered aqueous solution

form

buffered aqueous solution

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General description

Goat polyclonal anti-Mouse IgG (Fab specific) reacts with normal mouse serum, mouse IgG and the Fab fragment of mouse IgG, while no reaction is observed against the Fc fragment of mouse IgG. No reaction is observed with human IgG.
Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. An immunoglobulin has two heavy chains and two light chains connected by a disulfide bond. It mainly helps in immune defense. It is a glycoprotein. IgG is a major class of immunoglobulin. Mice possess five immunoglobulin classes- IgM, IgG, IgA, IgD and IgE. Mouse IgG is further divided into five classes- IgG1, IgG2a, IgG2b, IgG2c and IgG3.
Mouse IgG is a plasma B cell derived antibody isotype defined by its heavy chain. IgG is the most abundant antibody isotype found in mouse serum. IgG crosses the placental barrier, is a complement activator and binds to the Fc-receptors on phagocytic cells. The level of IgG may vary with the status of disease or infection.

Application

Anti-Mouse IgG (Fab specific) antibody produced in goat has been used in western blotting.
Goat polyclonal anti-Mouse IgG (Fab specific) may be used for the detection and quantitation of mouse IgG in serum and biological fluids. It may be used to monitor changes in IgG levels under various conditions such as infection. It may also be used when conjugated with a detector molecule as a secondary antibody.

Biochem/physiol Actions

Immunoglobulin G (IgG) participates in hypersensitivity type II and type III reactions. IgG2a has cytotropic properties and IgG2 molecules have the ability to activate the complement system. IgG helps in opsonization, complement fixation and antibody dependent cell mediated cytotoxicity.

Other Notes

Antibody adsorbed with human IgG.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Preparation Note

Adsorbed to reduce background with human samples.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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Yan-Wei Sha et al.
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Su M and Zhang Q
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Yuan D, et al.
Molecular Medicine Reports, 12(5), 7146-7152 (2015)
Wei-Wei Ni et al.
Molecular medicine reports, 16(3), 2887-2892 (2017-07-06)
Platanus acerifolia pollen is considered an important source of airborne allergens in numerous cities. Pla a 1 is a major allergen from P. acerifolia pollen. The present study aimed to express and purify Pla a 1, and to prepare its
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