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A2304

Sigma-Aldrich

Anti-Mouse IgG (Fab specific)–Peroxidase antibody produced in goat

affinity isolated antibody, buffered aqueous solution

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Synonym(s):
Goat Anti-Mouse IgG (Fab specific)- HRP
MDL number:

biological source

goat

Quality Level

conjugate

peroxidase conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

mouse

should not react with

bovine, horse, human

technique(s)

direct ELISA: 1:50,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200 using human tissue section
western blot: 1:80,000-1:160,000 using detecting β-actin in total cell extract of HeLa cells

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

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This Item
A3682A0168A3673
conjugate

peroxidase conjugate

conjugate

peroxidase conjugate

conjugate

peroxidase conjugate

conjugate

peroxidase conjugate

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

clone

polyclonal

clone

polyclonal

clone

polyclonal

clone

polyclonal

form

buffered aqueous solution

form

buffered aqueous solution

form

-

form

buffered aqueous solution

species reactivity

mouse

species reactivity

mouse

species reactivity

mouse

species reactivity

mouse

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General description

Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. An immunoglobulin has two heavy chains and two light chains connected by disulfide bond. It mainly helps in immune defense and is a glycoprotein. IgG is a major class of immunoglobulin. Immunoglobulin G (IgG) participates in hypersensitivity type II and type III. Mouse consists of five immunoglobulin classes- IgM, IgG, IgA, IgD and IgE. Mouse IgG is further divided into five classes- IgG1, IgG2a, IgG2b and IgG3. IgG helps in opsonization, complement fixation and antibody dependent cell mediated cytotoxicity.
Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases. Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3. IgG1 regulates complement fixation in mice.
Goat anti-mouse IgG (Fab specific)-peroxidase antibody associates with mouse IgG and mouse Fab fragment. The conjugate shows no reactivity with mouse Fc fragment, human IgG, IgA, IgM, kappa and lambda light chain, bovine IgG and IgM, or horse IgG.

Application

Murine IgG levels were quantitated in brain abscess homogenates by ELISA using HRP conjugated goat anti-mouse Fab specific antibody as the detection antibody.
Anti-Mouse IgG (Fab specific)–Peroxidase antibody has been used in western blotting.
Goat anti-mouse IgG (Fab specific)-peroxidase antibody is suitable for use in immunohistochemistry applications at 1:200 dilution using human tonsil tissues. The antibody can also be used for western blot assays at 1:80,000-1:160,000 dilutions using total cell extract of HeLa cells.

Other Notes

Antibody adsorbed with bovine, equine, and human serum proteins.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 0.05% MIT.

Preparation Note

Adsorbed to reduce background staining with bovine, horse and human samples.
Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2


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INK4a/Arf is required for suppression of EGFR/?EGFR (2-7)-dependent ERK activation in mouse astrocytes and glioma.
Lachat Y, et al.
Oncogene, 23(41), 6854?6863-6854?6863 (2004)
Investigating hsp gene expression in liver of Channa striatus under heat stress for understanding the upper thermal acclimation.
Purohit GK, et al.
BioMed Research International, 10-10 (2014)
Benjamin P Towler et al.
RNA biology, 12(7), 728-741 (2015-04-22)
Dis3 is a highly conserved exoribonuclease which degrades RNAs in the 3'-5' direction. Mutations in Dis3 are associated with a number of human cancers including multiple myeloma and acute myeloid leukemia. In this work, we have assessed the effect of
1, 25 (OH) 2-vitamin D3 actions on cell proliferation, size, gene expression, and receptor localization, in the HL-1 cardiac myocyte.
Nibbelink KA, et al.
The Journal of Steroid Biochemistry and Molecular Biology, 103(3-5), 533-537 (2007)
Gregory Hans et al.
Biochemical pharmacology, 69(5), 819-830 (2005-02-16)
Some synthetic ligands of the peripheral-type benzodiazepine receptor (PBR), an 18 kDa protein of the outer mitochondrial membrane, are cytotoxic for several tumor cell lines and arise as promising chemotherapeutic candidates. However, conflicting results were reported regarding the actual effect

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