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SAB3701171

Sigma-Aldrich

Anti-Mouse IgG1 (γ-chain specific)-Peroxidase antibody produced in rabbit

affinity isolated antibody, lyophilized powder

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Synonym(s):
HRP

biological source

rabbit

conjugate

peroxidase conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

lyophilized powder

species reactivity

mouse

technique(s)

immunohistochemistry: suitable
indirect ELISA: suitable
western blot: suitable

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

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SAB3701083SAB3701066SAB3701178
biological source

rabbit

biological source

rabbit

biological source

goat

biological source

rabbit

technique(s)

immunohistochemistry: suitable, western blot: suitable, indirect ELISA: suitable

technique(s)

immunohistochemistry: suitable, indirect ELISA: suitable, western blot: suitable

technique(s)

immunohistochemistry: suitable, indirect ELISA: suitable, western blot: suitable

technique(s)

immunohistochemistry: suitable, indirect ELISA: suitable, western blot: suitable

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

species reactivity

mouse

species reactivity

mouse

species reactivity

mouse

species reactivity

mouse

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

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General description

Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. It consists of a γ heavy chain in the constant (C) region. The monomeric 150kDa structure of IgG constitutes two identical heavy chains and two identical light chains with molecular weight of 50kDa and 25kDa, respectively. The primary structure of this antibody also contains disulfide bonds involved in linking the two heavy chains, linking the heavy and light chains and resides inside the chains. Maternal IgG is the only antibody transported across the placenta to the fetus. It passively immunizes the infants. IgG is further subdivided into four classes namely, IgG1, IgG2, IgG3, and IgG4 with different heavy chains, named γ1, γ2, γ3, and γ4, respectively. IgG1 is induced due to antibody responses to membrane proteins and soluble protein antigens. It is the most abundant subclass of IgG.

Specificity

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against Anti-Peroxidase, Anti-Rabbit Serum, Mouse IgG and Mouse Serum. Specificity was confirmed by ELISA against other Mouse or Human heavy chain isotypes. Cross reactivity with Mouse IgG2b and IgG3 is negligible (<0.5%). Cross reactivity with Mouse IgG2a and IgM is minimal.

Immunogen

Mouse IgG1 heavy chain

Application

Anti-Mouse IgG1 (γ-chain specific)-Peroxidase antibody produced in rabbit has been used in immunohistochemistry and Western blotting.

Physical properties

Antibody format: IgG

Physical form

Supplied in 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Reconstitution

Reconstitute with 1.0 mL deionized water (or equivalent).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

pictograms

Exclamation mark

signalword

Warning

hcodes

pcodes

Hazard Classifications

Skin Sens. 1

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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Epigenetic analysis of FHL1 tumor suppressor gene in human liver cancer
Jun Wang
Oncology Letters, 14(5), 6109-6116 (2017)
Dietary Protein to Carbohydrate Ratio and Caloric Restriction: Comparing Metabolic Outcomes in Mice
Samantha M.Solon-Biet
Cell Reports, 11(10), 1529-1534 (2015)
Line Barington et al.
Cells, 11(3) (2022-02-16)
B1 cells constitute a specialized subset of B cells, best characterized in mice, which is abundant in body cavities, including the peritoneal cavity. Through natural and antigen-induced antibody production, B1 cells participate in the early defense against bacteria. The G
Jun Wang et al.
Oncology letters, 14(5), 6109-6116 (2017-11-09)
Liver cancer is one of the most common types of cancer among human malignancies. Four and a half LIM domains 1 (FHL1), as a tumor suppressor gene, is frequently downregulated in multiple types of human cancer. However, the role and
Barbara Wróblewska et al.
Nutrients, 12(11) (2020-11-08)
There is no effective therapy for milk allergy. The role of lactic acid bacteria (LAB) and probiotics in protection against allergy-related outcomes is still under investigation. The aim of the study was to evaluate the immunomodulative and therapeutic potential of

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