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P3391

Millipore

Protein A-Sepharose from Staphylococcus aureus

lyophilized powder

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Synonym(s):
Protein A–Agarose
MDL number:
NACRES:
NA.56

form

lyophilized powder

Quality Level

packaging

glass bottle of 1 g
glass bottle of 1.5 g
glass bottle of 250 mg
glass bottle of 5 g

storage condition

do not freeze

extent of labeling

3 mg per mL

technique(s)

affinity chromatography: suitable

color

white to gray

matrix

Sepharose CL-4B

matrix activation

cyanogen bromide

matrix attachment

amino

matrix spacer

1 atom

capacity

≥16 mg/mL binding capacity (human IgG)

solubility

water: 1 g/L at 20 °C

suitability

conforms to structure for swelling capacity (1g swells to 4 to 5 mL)

storage temp.

2-8°C

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P7155P3296P3838
vibrant-m

P3296

Protein G Sepharose, Fast Flow

matrix

Sepharose CL-4B

matrix

-

matrix

Sepharose 4B Fast Flow

matrix

-

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

suitability

conforms to structure for swelling capacity (1g swells to 4 to 5 mL)

suitability

-

suitability

-

suitability

-

form

lyophilized powder

form

cell suspension

form

aqueous ethanol suspension

form

essentially salt-free, lyophilized powder

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

General description

Protein A-Sepharose when linked with antibody, helps improving antigen binding capacity of IgG crosslinked to column matrices. In this case, binding happens between Protein A and Fc portion of the IgG molecule leaving antigen specific sites free.

Application

Protein A-Sepharose was used:
  • for immunoprecipitated placentas for protein and RNA analyses using Western blot technique.
  • in chromatin immunoprecipitation to understand the link between Mediator and remodelling of chromatin structure at specific promoters.
  • as lysis buffer to remove endogenous G-type Igs in protein extraction of brain samples from female rats.
  • in immunoprecipitation of [32P] phosphomyristin C of rabbit antiserum, to study the down-regulation of protein kinase C (PKC) and phosphomyristin C (PMC) during T-cell development.
Protein A-Sepharose is used for affinity chromatography, antibody purification and characterization, immunoaffinity matrices, protein chromatography, protein A, G and L resins, and recombinant protein expression and analysis. Protein A-Sepharose has been used to develop strategies for investigating protein interactions, to improve the detection of celiac disease, and to study diabetes in children.

Quantity

Swelling: 1 g swells to approx. 4 ml.

Physical form

Supplied as lyophilized powder stabilized with lactose and dextran.

Legal Information

Sepharose is a trademark of Cytiva

related product

Product No.
Description
Pricing

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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Articles

Antibody fragmentation with our pepsin digestion protocol for IgG antibody fragmentation and preparation of F(ab’).

Protocols

To determine the molecular weights of protein antigens, to study protein/protein interactions, to determine specific enzymatic activity, to monitor protein post-translational modifications and to determine the presence and quantity of proteins.

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