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P2545

Millipore

Protein A–Agarose

saline suspension

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Synonym(s):
Protein A resin
MDL number:
NACRES:
NA.56

biological source

Staphylococcus aureus

Quality Level

form

saline suspension

extent of labeling

~2 mg per mL

matrix activation

cyanogen bromide

matrix attachment

amino

matrix spacer

1 atom

capacity

≥15 mg/mL binding capacity (human IgG)

storage temp.

2-8°C

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This Item
P9269P1925P5906
Millipore

P2545

Protein A–Agarose

Protein A–Agarose lyophilized powder

P9269

Protein A–Agarose

Millipore

P1925

Protein A–Agarose macrobeads

form

saline suspension

form

lyophilized powder

form

aqueous suspension

form

lyophilized powder

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

2-8°C

extent of labeling

~2 mg per mL

extent of labeling

~2 mg per mL

extent of labeling

~6 mg per mL

extent of labeling

~2 mg per mL

matrix activation

cyanogen bromide

matrix activation

cyanogen bromide

matrix activation

cyanogen bromide

matrix activation

cyanogen bromide

matrix attachment

amino

matrix attachment

amino

matrix attachment

-

matrix attachment

amino

General description

Protein A is a 42-kDa protein from Staphylococcus aureus and has five immunoglobulin binding domains.

Application

Protein A−Agarose has been used in the immunoprecipitation
  • of protein phosphatase and tensin homolog (PTEN) protein from S63 and NIH3T3 fibroblast cells
  • of prolyl hydroxylase domain 2 N27 cells
  • of Wnt3a and sonic hedgehog (Shh) in mouse fibroblast L-cells and human embryonic kidney (HEK) 293 cells

Protein A-agarose is used for affinity chromatography, antibody purification and characterization, and protein A, G and L resins. Protein A-agarose has been used to study the effects of protein A immunoadsorption in patients with chronic dilated cardiomyopathy as well as to study multiple sclerosis and gastric cancer.

Biochem/physiol Actions

Protein A binds to Fcγ (the constant region) and Fab (antigen binding fragment) of IgG. Protein A agarose resin is commercially used for affinity based purifications.

Physical form

Suspension in 0.5 M NaCl containing preservative

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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25G
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