MilliporeSigma
All Photos(1)

Documents

P9424

Millipore

ProteinA-Sepharose 4, Fast Flow from Staphylococcus aureus

aqueous ethanol suspension

Sign Into View Organizational & Contract Pricing

Synonym(s):
Protein A–Agarose
MDL number:
NACRES:
NA.56

biological source

Staphylococcus aureus

Quality Level

form

aqueous ethanol suspension

analyte chemical class(es)

proteins (Immunoglobulins of various mammalian species)

extent of labeling

~6 mg per mL

technique(s)

affinity chromatography: suitable

matrix

Sepharose 4B Fast Flow

matrix activation

cyanogen bromide

matrix attachment

amino

matrix spacer

1 atom

capacity

≥30 mg/mL binding capacity (human IgG)

storage temp.

2-8°C

Looking for similar products? Visit Product Comparison Guide

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
P3391P3296P7155
Millipore

P3296

Protein G Sepharose, Fast Flow

technique(s)

affinity chromatography: suitable

technique(s)

affinity chromatography: suitable

technique(s)

affinity chromatography: suitable

technique(s)

-

form

aqueous ethanol suspension

form

lyophilized powder

form

aqueous ethanol suspension

form

cell suspension

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

matrix

Sepharose 4B Fast Flow

matrix

Sepharose CL-4B

matrix

Sepharose 4B Fast Flow

matrix

-

analyte chemical class(es)

proteins (Immunoglobulins of various mammalian species)

analyte chemical class(es)

-

analyte chemical class(es)

proteins (Immunoglobulins of various mammalian species)

analyte chemical class(es)

-

General description

Protein A-Sepharose has been used to develop strategies for investigating protein interactions, to improve the detection of celiac disease, and to study diabetes in children.

Application

Protein A-Sepharose is used for affinity chromatography, antibody purification and characterization, immunoaffinity matrices, protein chromatography, protein A, G and L resins, and recombinant protein expression and analysis. Protein A-Sepharose has been used to develop strategies for investigating protein interactions, to improve the detection of celiac disease, and to study diabetes in children.
Protein A-Sepharose Fast Flow from Staphylococcus aureus has been used:
  • in co-immunoprecipitation assay
  • in immunodepletion
  • to purify IgG from human serum and plasma

Physical form

Suspension in 20% ethanol

Legal Information

Sepharose is a trademark of Cytiva

pictograms

Flame

signalword

Warning

hcodes

Hazard Classifications

Flam. Liq. 3

Storage Class

3 - Flammable liquids

wgk_germany

WGK 3

flash_point_c

40 °C


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Customers Also Viewed

Slide 1 of 6

1 of 6

Protein A–Agarose lyophilized powder

Millipore

P1406

Protein A–Agarose

Protein A Agarose >98% (HPLC and SDS-PAGE), suspension

Roche

PROTAA-RO

Protein A Agarose

Protein G–Agarose lyophilized powder, Contains lactose stabilizers that must be removed prior to use.

Millipore

P7700

Protein G–Agarose

Protein A-Sepharose™ CL-4B Cytiva 17-0780-01, pack of 1.5 g

GE17-0780-01

Protein A-Sepharose CL-4B

Protein A Sepharose™ Cl-4B Cytiva 17-0963-02, pack of 500 mL

GE17-0963-02

Protein A Sepharose Cl-4B

Protein A Sepharose™ Cl-4B Cytiva 17-0963-03, pack of 25 mL

GE17-0963-03

Protein A Sepharose Cl-4B

Joyce A Benjamins et al.
Neurology(R) neuroimmunology & neuroinflammation, 6(3), e550-e550 (2019-05-03)
To identify whether factors toxic to oligodendrocytes (OLs), released by B cells from patients with MS, are found in extracellular microvesicles enriched in exosomes. Conditioned medium (Sup) was obtained from cultures of blood B cells of patients with MS and
Yuhua Zhang et al.
Plant physiology, 149(4), 1860-1871 (2009-02-06)
Trehalose-6-phosphate (T6P) is a proposed signaling molecule in plants, yet how it signals was not clear. Here, we provide evidence that T6P functions as an inhibitor of SNF1-related protein kinase1 (SnRK1; AKIN10/AKIN11) of the SNF1-related group of protein kinases. T6P
H Sternlicht et al.
Proceedings of the National Academy of Sciences of the United States of America, 90(20), 9422-9426 (1993-10-15)
A role in folding newly translated cytoskeletal proteins in the cytosol of eukaryotes has been proposed for t-complex polypeptide 1 (TCP1). In this study, we investigated tubulin and actin biogenesis in Chinese hamster ovary (CHO) cells. When extracts of pulse-labeled
Mazhar Adli et al.
Nature protocols, 6(10), 1656-1668 (2011-10-01)
Chromatin immunoprecipitation (ChIP) combined with high-throughput sequencing (ChIP-seq) has become the gold standard for whole-genome mapping of protein-DNA interactions. However, conventional ChIP protocols necessitate the use of large numbers of cells, and library preparation steps associated with current high-throughput sequencing
Lydia Alvarez-Erviti et al.
Neurobiology of disease, 42(3), 360-367 (2011-02-10)
Alpha-synuclein aggregation plays a central role in Parkinson's disease pathology. Direct transmission of alpha-synuclein from pathologically affected to healthy unaffected neurons may be important in the anatomical spread of the disease through the nervous system. We have demonstrated that exosomes

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service