추천 제품
생물학적 소스
potato
유형
Grade III
양식
lyophilized powder (partially purified)
ATPase activity
≥60 units/mg protein
분자량
~49 kDa by gel filtration
구성
Protein, 30-60%
solubility
H2O: soluble 10 mg/mL, slightly hazy
응용 분야
diagnostic assay manufacturing
외래 활성
Acid Phosphatase ≤5% of base activity
저장 온도
−20°C
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애플리케이션
Apyrase is used to hydrolyze nucleoside triphosphates and diphosphates. For hydrolysis of organic di and triphosphates, the optimal pH is 6, and for inorganic substrates, the optimal pH is 5.1[1] Apyrase, from Sigma, has been used in inhibition studies of platelet-aggregation.[2] The enzyme has been used to assess the possibility of ATP involvement in intestinal lamina propria Th17 cell differentiation.[3]
At least two isoenzymes are found in different varieties of S. tuberosum: one with a high ATPase/ADPase ratio (∼10) and another with a low ratio (∼1).
Reaction: ATP → ADP+Pi → AMP+2Pi.
Reaction: ATP → ADP+Pi → AMP+2Pi.
생화학적/생리학적 작용
Apyrase is found in all eukaryotes and some prokaryotes. Apyrase, from potato, has a crucial role in regulating growth and development. Apyrase is involved in the inactivation of synaptic ATP as a neurotransmitter following nerve stimulation and in the inhibition of ADP induced platelet aggregation to prevent thrombosis.[4] Divalent metal ions are required for activity and best activity is observed with calcium ion at 5 mM. The molecular weight of the protein is found to be approximately 45 kDa.
포장
Sold on the basis of ATPase units
기타 정보
Mixture of high and low ratio isoenzymes.
품질
Contains ≤ 5% acid phosphatase.
단위 정의
One unit will liberate 1.0 μmole of inorganic phosphate from ATP or ADP per min at pH 6.5 at 30 °C.
신호어
Danger
유해 및 위험 성명서
예방조치 성명서
Hazard Classifications
Resp. Sens. 1
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
개인 보호 장비
Eyeshields, Gloves, type N95 (US)
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시험 성적서(COA)
Lot/Batch Number
이미 열람한 고객
Yen-Ta Chen et al.
Journal of tissue engineering and regenerative medicine, 13(12), 2155-2168 (2019-09-11)
This study tested the hypothesis that preactivated and disaggregated shape-changed platelet (PreD-SCP) therapy significantly protected rat kidney from ischemia-reperfusion (IR) injury. Adult-male Sprague-Dawley rats (n = 24) were equally categorized into Groups 1 (sham-operated control [SC]), 2 (SC + PreD-SCP)
Marko Gosak et al.
Life (Basel, Switzerland), 11(5) (2021-05-01)
Ca2+ homeostasis and signaling disturbances are associated with lens pathophysiology and are involved in cataract formation. Here, we explored the spatiotemporal changes in Ca2+ signaling in lens epithelial cells (LECs) upon local mechanical stimulation, to better understand the LECs' intercellular
H J Kruse et al.
Arteriosclerosis and thrombosis : a journal of vascular biology, 14(11), 1821-1828 (1994-11-01)
The blocker of receptor-mediated calcium entry SK&F 96365 was used to evaluate the contribution of calcium influx to the formation of biologically active endothelial prostanoids and endothelium-derived relaxing factor (EDRF). SK&F 96365 inhibited histamine-stimulated calcium entry into human umbilical vein
Properties of Two Apyrases from Solanum tuberosum
Kettlun, A. et al.
Phytochemistry, 21(3), 551-558 (1982)
Xiao-Jing Kuang et al.
Theranostics, 12(8), 3703-3718 (2022-06-07)
Rationale: Stress is a major risk factor for the development of depression. However, the underlying molecular mechanisms of stress vulnerability in depression are largely uncharacterized. Methods: P2X2 receptors (a major receptor for gliotransmitter-ATP) in the medial prefrontal cortex (mPFC) were
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