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A6535

Sigma-Aldrich

Apyrase from potatoes

ATPase ≥200 units/mg protein, lyophilized powder

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Synonym(s):
Adenosine 5′-diphosphatase, Adenosine 5′-triphosphatase
CAS Number:
Enzyme Commission number:
EC Number:
MDL number:
NACRES:
NA.54

type

Grade VII

Quality Level

form

lyophilized powder

ATPase activity

≥200 units/mg protein

secondary activity

≥50 % of base activity ADPase

composition

Protein, 25-60%

solubility

H2O: soluble 1.0 mg/mL

application(s)

diagnostic assay manufacturing

foreign activity

Acid Phosphatase ≤2% of base activity

storage temp.

−20°C

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This Item
SRE0019A6410A6132
Apyrase from potatoes ATPase ≥200 units/mg protein, lyophilized powder

Sigma-Aldrich

A6535

Apyrase from potatoes

Sigma-Aldrich

Sigma-Aldrich

A6410

Apyrase from potatoes

Apyrase from potatoes ATPase ≥3.0 units/mg protein, lyophilized powder

Sigma-Aldrich

A6132

Apyrase from potatoes

solubility

H2O: soluble 1.0 mg/mL

solubility

H2O: soluble 1.0 mg/mL

solubility

H2O: soluble 1.0 mg/mL

solubility

-

application(s)

diagnostic assay manufacturing

application(s)

diagnostic assay manufacturing

application(s)

-

application(s)

-

foreign activity

Acid Phosphatase ≤2% of base activity

foreign activity

Acid Phosphatase ≤2% of base activity

foreign activity

Acid Phosphatase ≤2% of base activity

foreign activity

-

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

Quality Level

300

Quality Level

400

Quality Level

200

Quality Level

300

Application

Apyrase is used to hydrolyze nucleoside triphosphates and diphosphates. For hydrolysis of organic di and triphosphates, the optimal pH is 6, and for inorganic substrates, the optimal pH is 5.1. Apyrase, from Sigma, has been used in inhibition studies of platelet-aggregation .
At least two isoenzymes are found in different varieties of S. tuberosum: one with a high ATPase/ADPase ratio (∼10) and another with a low ratio (∼1).
Reaction: ATP → ADP+Pi → AMP+2Pi.

Biochem/physiol Actions

Apyrase is found in all eukaryotes and some prokaryotes. Apyrase, from potato, has a crucial role in regulating growth and development. Apyrase is involved in the inactivation of synaptic ATP as a neurotransmitter following nerve stimulation and in the inhibition of ADP induced platelet aggregation to prevent thrombosis . Divalent metal ions are required for activity and best activity is observed with calcium ion at 5 mM.

Packaging

Sold on the basis of ATPase units.

Other Notes

This grade has a low ATPase/ADPase ratio.

Unit Definition

One unit will liberate 1.0 μmole of inorganic phosphate from ATP or ADP per min at pH 6.5 at 30 °C.

Physical form

Partially purified, lyophilized powder containing potassium succinate buffer salts.

Preparation Note

Derived from red potato

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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25G
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705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

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