C1999

CMP-Sialic Acid Synthetase from Neisseria meningitidis group B

recombinant, expressed in E. coli BL21, ≥10 units/mg protein

• 동의어(들): CTP: N-Acylneuraminate cytidylyltransferase
• 효소 위원회 번호: 2.7.7.43 (BRENDA, IUBMB)
• MDL number: MFCD01323074
• UNSPSC 코드: 12352204
• NACRES: NA.54

속성

• 재조합: expressed in E. coli BL21
• Quality Level: 200
• 양식: lyophilized solid
• 특이 활성도: ≥10 units/mg protein
• 분자량: 26.0 kDa
• 배송 상태: dry ice
• 저장 온도: −20°C

설명

일반 설명

Cytidine monophosphate (CMP)-Sialic Acid Synthetase from Neisseria meningitidis group B is encoded in neuA gene. The protein has a molecular weight of 24.8 kDa.[1]

애플리케이션

The enzyme has been utilized to synthesize CMP-sialic acid and its derivatives.[2]

생화학적/생리학적 작용

Cytidine monophosphate (CMP)-sialic acid synthetase catalyses the conversion of N?acetylneuraminic acid (NeuNAc) to CMP-NeuNAc.[1] CMP-sialic acid synthetase has globular α/β domain and is categorised under αβα three-layered sandwich fold. The dimerization domain aids the interaction between the monomers. It also has mononucleotide binding and NeuAc binding pocket.[3] Mg²⁺ is essential for the catalytic functionality of CMP-sialic acid synthetase.[4]

단위 정의

One unit will catalyze the formation of 1 μmol CMP-Neu-5-Ac from Neu-5-Ac and CTP per minute at 37 °C at pH 8.0.

물리적 형태

Supplied as a lyophilized powder containing Tris-HCl and NaCl.

분석 메모

Enzymatic activity assays are performed in Tris-HCl buffer (100 mM, pH 8.5) containing Neu-5-Ac (1 mM) and CTP (1 mM) at 37 °C for 30 min and analyzed using capillary electrophoresis with a UV detector (200 nm).

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable