DNTP100

Deoxynucleotide Set, 100 mM

Individual dNTPs for routine PCR; 0.25 mL each

• 동의어(들): DNTP Set for PCR
• UNSPSC 코드: 41106305
• NACRES: NA.52

속성

• Quality Level: 200
• 양식: liquid
• 농도: 100 mM
• 색상: colorless
• 배송 상태: dry ice
• 저장 온도: −20°C

설명

일반 설명

Deoxynucleotide triphosphates (dNTPs) are organic molecules, which contains nucleobase bound to deoxyribose. This dNTPs set includes 0.25 mL each of 100mM aqueous solutions of four deoxyribonucleotides, deoxyribose adenosine triphosphate (dATP), deoxyribose cytidine triphosphate (dCTP), deoxyribose guanosine triphosphate (dGTP), and deoxyribose thymidine triphosphate (dTTP) in separate tubes for convenience and easy PCR reaction setup.

애플리케이션

Deoxynucleotide Set, 100 mM has been used as a component of the reaction mixture for polymerase chain reaction (PCR).It has also been used as a component of the reaction mixture for amplification refractory mutation system polymerase chain reaction (ARMS-PCR) to genotype single-nucleotide polymorphisms (SNPs).

Deoxynucleotide Set, 100 mM has been used:

• to biotinylate λ-DNA for magnetic tweezers measurements
• in RAPD PCR
• in FISH probe generation and labeling
• in PCR amplification of genomic DNA
• in multiplex PCR
• in first-strand cDNA synthesis
• in TUNEL labeling
• in RT-PCR
• as a component of the reaction mixture for polymerase chain reaction (PCR)

생화학적/생리학적 작용

Deoxynucleotide triphosphates (dNTPs) are substrates for DNA polymerizing enzymes. They act as building blocks of nucleic acid molecules and are acidic in nature. dNTPs are key components of PCR master mixes and are highly essential to amplify DNA. Optimal levels of dNTPs ensure proper chromosomal replication and in turn maintain genome stability.

특징 및 장점

• Provided as 4 separate tubes of dNTPs.
• Allows optimization of individual dNTP concentrations.

포장

0.25 mL each of 100 mM dATP, dCTP, dGTP and dTTP

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable