G6751

α-Glycerophosphate Dehydrogenase from rabbit muscle

Type I, ammonium sulfate suspension, 100-300 units/mg protein

• 동의어(들): sn-Glycerol-3-phosphate Dehydrogenase from rabbit muscle, sn-Glycerol-3-phosphate:NAD⁺ 2-oxidoreductase
• CAS Number: 9075-65-4
• 효소 위원회 번호: 1.1.1.8 (BRENDA, IUBMB)
• EC Number: 232-982-3
• MDL number: MFCD00130487
• UNSPSC 코드: 12352204
• NACRES: NA.54

속성

• 생물학적 소스: rabbit muscle
• Quality Level: 200
• 유형: Type I
• 양식: ammonium sulfate suspension
• 특이 활성도: 100-300 units/mg protein
• 저장 조건: (Tightly closed)
• 기술: activity assay: suitable
• 색상: white
• 외래 활성: Lactic dehydrogenase, pyruvate kinase, aldolase, and glyceraldehyde-3-phosphate dehydrogenase ≤0.01%; Triosephosphate isomerase ≤0.02%
• 저장 온도: 2-8°C

설명

일반 설명

α-Glycerophosphate dehydrogenase is a membrane protein and a flavin-linked primary dehydrogenase.[1]

Research area: Cell Signaling[1]

애플리케이션

α-Glycerophosphate Dehydrogenase from rabbit muscle has been used:

• in the reaction mixture to measure the glycerol kinase activity[2]
• to demonstrate compartmentalized enzymatic reactions where NADH is involved[3]
• in the reaction mixture assay for L-fuculose-1-phosphate aldolase[4]

생화학적/생리학적 작용

α-Glycerophosphate dehydrogenase catalyzes the oxidation of L-α-glycerophosphate (Glp) to form dihydroxyacetone phosphate (DHAP) and hydrogen peroxide (H2O2).[5] This enzyme is associated with respiratory electron transport chain, glycolysis, and phospholipid metabolism.[1]

단위 정의

One unit will convert 1.0 μmole of dihydroxyacetone phosphate to α-glycerophosphate per min at pH 7.4 at 25 °C.

물리적 형태

Crystalline suspension in 3.2 M (NH₄)₂SO₄ and 0.1 g/L EDTA solution, pH 6.0

제조 메모

Prepared by modification of the method of Fondy.

분석 메모

Protein determined by biuret.

안전성 정보

• 픽토그램: GHS08
• 신호어: Danger
• 유해 및 위험 성명서: H334
• 예방조치 성명서: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable