추천 제품
일반 설명
Lysozyme is present in tears, saliva and human milk. The human lysozyme expressed in rice grains is an excellent supplemental source of this antimicrobial protein. Lysozyme is mapped to human chromosome 12q15 and corresponds to a molecular weight of 16.5kDa. It is a positively charged and a C-type lysozyme with α and β structural domains.
애플리케이션
Lysozyme human has been used:
- in the interaction studies with polyphenon-60 by calorimetric and spectroscopic techniques
- in the drug release assay of dexamethasone (DEX) - carbon nanotube based delivery system
- in the surface plasmon resonance (SPR) for measuring its interaction with human angiotensin I-converting enzyme (ACE)
생화학적/생리학적 작용
Lysozyme is an antibacterial enzyme, which acts on the peptidoglycan layer at the on the N-acetylgluosamine (NAG) and N-acetylmuramic acid linkage, resulting in the lysis of microbial cells. It is a potential anti-inflammatory factor and also has anti-fungal and anti-viral functionality. In inflammatory bowel disease, lysozyme is highly expressed in colonic tissue. Point mutations in lysozyme is implicated in the pathogenesis of hereditary non-neuropathic renal amyloidosis.
특징 및 장점
Lysobac™ is a breakthrough for bacterial cell lysis and can be used in diagnostic applications, bioprocessing and life science research. It is recombinant human lysozyme produced in an animal-free production system. Animal-free production eliminates the safety risk and inconsistent lot-to-lot performance of hen egg white lysozyme. Lysobac™ has significantly higher bioactivity than hen egg white lysozyme. One gram replaces 4 grams of hen egg white lysozyme. It also delivers gentle cell lysis, unlike mechanical lysis which can cause protein shear and reduce protein yields by up to 20%.
Advantages:
Advantages:
- Improves Bacterial Fermentation Efficiency
- Greater bacterial lysis activity per mg.
- 1 gram of delivers more lysis activity than 4 grams of HEWL
- Animal-free and consistent leading to improved regulatory efficiency for drug manufacturing
- Improved recombinant protein yield compared to mechanical lysis
- Reduces cost of goods
포장
Package size based on protein content
단위 정의
One unit will lyse 0.6 μg of Micrococcus lysodeikticus per minute by turbidimetric detection at 600 nm when suspended in buffer at pH 6.2 at 25 °C.
기타 정보
View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer
법적 정보
Lysobac is a trademark of InVitria
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
이미 열람한 고객
Suppression of human arthritis synovial fibroblasts inflammation using dexamethasone-carbon nanotubes via increasing caveolin-dependent endocytosis and recovering mitochondrial membrane potential
Lee YK, et al.
International journal of nanomedicine, 12(5), 5761-5761 (2017)
Inflammatory bowel disease: an impaired barrier disease
Jager S, et al.
Langenbeck'S Archives of Surgery / Deutsche Gesellschaft Fur Chirurgie, 398(1), 1-12 (2013)
A novel lysozyme mutation Phe57Ile associated with hereditary renal amyloidosis
Yazaki M, et al.
Kidney International, 63(5), 1652-1657 (2003)
Lysozyme and bilirubin bind to ACE and regulate its conformation and shedding
Danilov SM, et al.
Scientific reports, 6(5), 34913-34913 (2016)
Huahua He et al.
Frontiers in bioengineering and biotechnology, 8, 118-118 (2020-03-27)
Human lysozyme (hLYZ), known for its bacteriolytic activity, is widely applied in the food and pharmaceutical industries as an antimicrobial agent. However, its extensive application was limited by its low large-scale production efficiency. In this study, a combinational method of
프로토콜
This enzymatic rate determination may be used for Lysozyme products. It is not to be used to assay recombinant or insoluble Lysozyme on agarose.
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