L3382

Lectin from Helix pomatia

lyophilized powder, salt, free

• 동의어(들): Helix pomatia agglutinin, HPA
• UNSPSC 코드: 12352202
• NACRES: NA.32

속성

• 양식: lyophilized powder
• Quality Level: 200
• 효능: <4 μg per mL agglutination activity
• 불순물: salt, free
• 저장 온도: −20°C

설명

일반 설명

Lectin from Helix pomatia is a hexameric glycoprotein with a molecular mass of 79 kDa. It issecreted by the albumen gland of the roman snail. Helix pomatia agglutinin (HPA) is specific for N-acetylgalactosamine (GalNAc).

애플리케이션

Lectin from Helix pomatia has been used to conjugate with biotin and acridinium and used in lectin-agglutination tests.

생화학적/생리학적 작용

HPA has anti-A human blood group specificity and has an affinity for terminal N-acetyl-α-D-galactosaminyl residues. Conjugates are prepared from affinity purified lectin.

Lectin from Helix pomatia protects fertilized eggs by coating them with perivitelline fluid. They exhibit antibacterial activities and immunity in snail. Helix pomatia agglutinin (HPA) possess the potential to agglutinate pathogens. HPA has also emerged as a marker of altered Oglycosylation in cancer.

제조 메모

Affinity purified by a modification of the method of Hammarstrom and Kabat.

분석 메모

Where reported, agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a 2% suspension of human blood group A erythrocytes after 1 hour incubation at 25 °C.

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)