추천 제품
사용
sufficient for 100 colorimetric tests
입력
cell
plasma
serum
solution(s) (collagen)
검출 방법
colorimetric
관련 질환(들)
endocrinological disorders, diabetes; cancer
저장 온도
−20°C
일반 설명
Lactate is generated by lactate dehydrogenase (LDH) under hypoxic or anaerobic conditions. Monitoring lactate levels is, therefore, a good indicator of the balance between tissue oxygen demand and utilization and is useful when studying cellular and animal physiology. Simple, direct and automation-ready procedures for measuring lactate concentration are very desirable.
애플리케이션
The L-Lactate Assay Kit may be used for:
- Cancer Research
- Diabetes Research
- Endocrinological Disease Research
특징 및 장점
Sensitive Detection Range: Detect L-Lactate levels accurately across a wide range, from 0.05 mM to 2 mM, using only 20 μL of sample in a 96-well plate setup, ensuring highly sensitive analysis with minimal sample requirement.
Simplified Process: Experience a streamlined process with the addition of only a single working reagent and a 20 minute room temperature reaction, reducing complexity and saving valuable time and effort.
Compatibility with High-Throughput Systems: Easily incorporate our kit into high-throughput handling systems, ensuring smooth and accurate processing, enhancing efficiency in your laboratory workflow.
Simplified Process: Experience a streamlined process with the addition of only a single working reagent and a 20 minute room temperature reaction, reducing complexity and saving valuable time and effort.
Compatibility with High-Throughput Systems: Easily incorporate our kit into high-throughput handling systems, ensuring smooth and accurate processing, enhancing efficiency in your laboratory workflow.
적합성
Suitable for lactate determination in serum, plasma, and cell media samples.
원리
The Lactate Assay Kit is based on lactate dehydrogenase catalyzed oxidation of lactate, in which the formed NADH reduces a formazan (MTT) reagent. The intensity of the product color, measured at 565 nm, is proportionate to the lactate concentration in the sample.
기타 정보
For additional information on our range of Biochemicals, please complete this form.
Storage Class Code
10 - Combustible liquids
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시험 성적서(COA)
Lot/Batch Number
Triiodothyronine prevents tissue hypoxia in experimental sepsis: potential therapeutic implications.
Iordanis S Mourouzis et al.
Intensive care medicine experimental, 9(1), 17-17 (2021-04-10)
Ana Zúñiga et al.
ACS synthetic biology, 10(12), 3527-3536 (2021-12-02)
Bacteria equipped with genetically encoded lactate biosensors are promising tools for biopharmaceutical production, diagnostics, and cellular therapies. However, many applications involve glucose-rich and anoxic environments, in which current whole-cell lactate biosensors show low performance. Here we engineer an optimized, synthetic
Katharina Richard et al.
The Journal of experimental medicine, 218(2) (2020-11-21)
Two cosegregating single-nucleotide polymorphisms (SNPs) in human TLR4, an A896G transition at SNP rs4986790 (D299G) and a C1196T transition at SNP rs4986791 (T399I), have been associated with LPS hyporesponsiveness and differential susceptibility to many infectious or inflammatory diseases. However, many
Tia R Tidwell et al.
Cancer & metabolism, 10(1), 9-9 (2022-05-17)
Most in vitro cancer cell experiments have been performed using 2D models. However, 3D spheroid cultures are increasingly favored for being more representative of in vivo tumor conditions. To overcome the translational challenges with 2D cell cultures, 3D systems better
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