MAK464

Lactate Dehydrogenase Assay Kit

sufficient for 100 colorimetric tests

• Synonym(s): LDH Assay Kit, Lactate Dehydrogenase Activity Assay Kit
• UNSPSC Code: 12352204
• NACRES: NA.84

Properties

• Quality Level: 100
• application(s): pharmaceutical
• detection method: colorimetric
• relevant disease(s): cancer; hematological disorder
• shipped in: wet ice
• storage temp.: −20°C

Description

General description

Lactate dehydrogenase (LDH) is an oxidoreductase which catalyzes the interconversion of lactate and pyruvate. When disease or injury affects tissues containing LDH, the cells release LDH into the bloodstream, where it is identified in higher than normal levels. Therefore, LDH is most often measured to evaluate the presence of tissue or cell damage.

Features and Benefits

• Linear detection range 2-200 U/L
• Compatible with high-throughput handling systems.
• Simple procedure allows for reliable quantitation of LDH activity within 30 minutes.

Suitability

The kit is suitable for lactate dehydrogenase activity determination in serum, plasma, and other sources. It is also suitable for the characterization and quality control of LDH production, as well as for the screening and evaluation of LDH modulators in drug discovery.

Principle

The non-radioactive colorimetric LDH assay is based on the reduction of the tetrazolium salt MTT in a NADH-coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at 565 nm. The intensity of the purple color formed is directly proportional to the enzyme activity.

Safety Information

• pictograms: GHS02,GHS05,GHS07
• signalword: Danger
• hcodes: H225,H290,H315,H319,H336
• pcodes: P210 - P233 - P234 - P240 - P303 + P361 + P353 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Flam. Liq. 2 - Met. Corr. 1 - Skin Irrit. 2 - STOT SE 3
• target_organs: Respiratory system
• Storage Class: 3 - Flammable liquids
• wgk_germany: WGK 2