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ECM002

Sigma-Aldrich

Thrombospondin-1 human

recombinant, expressed in HEK 293 cells, lyophilized powder, suitable for cell culture

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Synonym(s):
THBS, THBS1, TSP, TSP1, Thrombospondin-1
NACRES:
NA.75

biological source

human

Quality Level

recombinant

expressed in HEK 293 cells

sterility

sterile

assay

≥95% (SDS-PAGE)

form

lyophilized powder

mol wt

127.5 kDa (The protein migrates as a 140 kDa band on SDS-PAGE due to glycosylation.)

packaging

pkg of 50 μg

technique(s)

cell culture | mammalian: suitable

impurities

≤1 EU/μg endotoxin, tested

solubility

water: soluble

NCBI accession no.

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... THBS1(7057)

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This Item
SAB1401385SAE0077SAE0075
vibrant-m

ECM002

Thrombospondin-1 human

vibrant-m

SAE0077

MMP-9 human

sterility

sterile

sterility

-

sterility

-

sterility

-

biological source

human

biological source

mouse

biological source

human

biological source

-

assay

≥95% (SDS-PAGE)

assay

-

assay

95% (SDS-PAGE)

assay

≥95% (SDS-PAGE)

Quality Level

200

Quality Level

100

Quality Level

200

Quality Level

200

form

lyophilized powder

form

buffered aqueous solution

form

liquid

form

lyophilized powder

General description

Thrombospondin-1 (TSP1) belongs to family of glycoproteins and possesses a complex trimeric structure. The structure comprises a heparin-binding domain and a procollagen homology domain located at the amino terminus, while the carboxyl-terminal end contains type I, II, and III repeats. This protein is manufactured in human cells using an all-human production system, with no serum. The human cells expression system allows human-like glycosylation and folding, and often supports better stability of the protein in culture. Recombinant human thrombospondin-1 is expressed in HEK293 cells as a glycoprotein with a calculated moleculaer mass of 127.5 kDa.

Application

Thrombospondin 1 human (TSP1) has been used:

  • To treat hepatocyte (IHH) cancer cells to study the role of aspartyl protease 1 (MfSAP1) in extracellular matrix degradation.
  • In in vitro Extracellular Matrix (ECM) protein degradation assays.
  • In rat C6 astroglioma cells, for coating the cell culture plates to study the effects of integrins on CNTF (ciliary neurotrophic factor) expression.
  • In the preparation of secreted factor cocktail for analysis of the human mesenchymal stem cell secretome.
    Recommended for use as a cell culture substratum at 1-5 μg/cm2 or 0.1-25 μg/ml. Optimal concentration depends on cell type as well as the application or research objectives.

Biochem/physiol Actions

Thrombospondin (TSP1) plays a crucial role in guiding extracellular matrix (ECM) synthesis and facilitating dynamic tissue remodeling. It is involved in various biological processes including angiogenesis, matrix assembly, activation of transforming growth factor (TGF), and promotion of neurite outgrowth. TSP1 binds to various cell surface receptors, such as integrins and integrin-associated protein CD47. Studies have also shown that the signaling pathway involving TSP1-CD47 promotes the generation of reactive oxygen species (ROS) in human pulmonary artery endothelial cells. This signaling pathway plays a significant role in the manifestation of sickle cell-associated vasculopathy and the subsequent progression of pulmonary hypertension. Moreover, hypoxia-inducible factor (HIF)-2α has been shown to upregulate the expression of TSP1 under hypoxic conditions. TSP1 also plays a crucial role in inflammatory processes and post-inflammatory tissue dynamics. It is upregulated in rheumatoid synovial tissues and might be associated with rheumatoid arthritis.

Features and Benefits

  • Human-derived thrombospondin
  • Produced through recombinant expression in HEK 293 cells
  • Low endotoxin levels

Physical form

Supplied as a powder, lyophilized from phosphate buffered saline.

Analysis Note

The biological activity of recombinant human thrombospondin-1 was tested in culture by measuring the ability of immobilized DTT-treated thrombospondin-1 to support adhesion of SVEC4-10 cells.

hcodes

Hazard Classifications

Aquatic Chronic 3

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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Functions of Thrombospondin-1 in the Tumor Microenvironment
Kaur S, et al.
International Journal of Molecular Sciences, 22(9), 4570-4570 (2021)
Identification of Malassezia furfur Secreted Aspartyl Protease 1 (MfSAP1) and Its Role in Extracellular Matrix Degradation
Si En Poh, et al.
Frontiers in Cellular and Infection Microbiology, 10 (2020)
Thrombospondin-1 CD47 Signalling: From Mechanisms to Medicine
Kale A, et al.
International Journal of Molecular Sciences, 22(8), 4062-4062 (2021)
Artificial Extracellular Matrices to Functionalize Biomaterial Surfaces
Bierbaum S, et al.
Comprehensive Biomaterials, 2 (2017)
Runt related transcription factor-1 plays a central role in vessel co-option of colorectal cancer liver metastases
Rada M, et al.
Communications biology, 4(1), 950-950 (2021)

Articles

The extracellular matrix (ECM) and its attachment factor components are discussed in this article in relation to their function in structural biology and their availability for in vitro applications.

The extracellular matrix (ECM) is secreted by cells and surrounds them in tissues.

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