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AA0100

Sigma-Aldrich

Ammonia Assay Kit

sufficient for 100 assays

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NACRES:
NA.84

usage

sufficient for 100 assays

storage temp.

2-8°C

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IP50ET0300CS1000
Ammonia Assay Kit sufficient for 100 assays

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AA0100

Ammonia Assay Kit

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ET0300

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storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

Application

Ammonia concentration was measured in serum and urine using the Sigma Ammonia Assay Kit in a study assessing expression of renal ammonia transporters in response to chromic metabolic acidosis.
This kit is for the quantitative, enzymatic determination of ammonia in food and biological samples. Ammonia reacts with α-ketoglutaric acid (KGA) and reduced nicotinamide adenine dinucleotide phosphate (NADPH) in the presence of L-glutamate dehydrogenase (GlDH) to form L-glutamate and oxidized nicotinamide adenine dinucleotide phosphate (NADP+). The decrease in absorbance at 340 nm, due to the oxidation of NADPH, is proportional to the ammonia concentration. L-Glutamate dehydrogenase reacts specifically with ammonia. The Ammonia Assay Kit may be used to determine ammonia concentrations in the range of 0.2-15 μg/ml.

Kit Components Only

Product No.
Description

  • Ammonia Assay Reagent 10 vial(s)

  • L-Glutamate Dehydrogenase for Ammonia Assay 1 mL/vial

  • Ammonia Standard Solution 1 mL/vial

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Tingting Xiang et al.
Nature communications, 11(1), 108-108 (2020-01-09)
In cnidarian-Symbiodiniaceae symbioses, algal endosymbiont population control within the host is needed to sustain a symbiotic relationship. However, the molecular mechanisms that underlie such population control are unclear. Here we show that a cnidarian host uses nitrogen limitation as a
Junpeng Jiang et al.
BMC plant biology, 19(1), 239-239 (2019-06-07)
Ammonium transporters (AMTs), a family of proteins transporting ammonium salt and its analogues, have been studied in many aspects. Although numerous studies have found that ammonium affects the interaction between plants and pathogens, the role of AMTs remains largely unknown
Bergmeyer, H.U., and Beutler, H-O.
Methods of Enzymatic Analysis, 454-461 (1985)
Renal expression of the ammonia transporters, Rhbg and Rhcg, in response to chronic metabolic acidosis.
Seshadri, R.
American Journal of Physiology: Renal Physiology, 290, F397-F408 (2006)
Na Gao et al.
Fish & shellfish immunology, 65, 226-234 (2017-04-22)
Ammonia is highly toxic to aquatic organisms, but whether ammonia excretion or ammonia metabolism to less toxic compounds is the major strategy for detoxification in marine fish against chronic ammonia exposure is unclear to date. In this study, we investigated

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