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AA0100

Sigma-Aldrich

Ammonia Assay Kit

sufficient for 100 assays

Synonym(s):

Ammonia Quantitative Test Kit

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.84

usage

sufficient for 100 assays

storage temp.

2-8°C

Application

Ammonia concentration was measured in serum and urine using the Sigma Ammonia Assay Kit in a study assessing expression of renal ammonia transporters in response to chromic metabolic acidosis.
This kit is for the quantitative, enzymatic determination of ammonia in food and biological samples. Ammonia reacts with α-ketoglutaric acid (KGA) and reduced nicotinamide adenine dinucleotide phosphate (NADPH) in the presence of L-glutamate dehydrogenase (GlDH) to form L-glutamate and oxidized nicotinamide adenine dinucleotide phosphate (NADP+). The decrease in absorbance at 340 nm, due to the oxidation of NADPH, is proportional to the ammonia concentration. L-Glutamate dehydrogenase reacts specifically with ammonia. The Ammonia Assay Kit may be used to determine ammonia concentrations in the range of 0.2-15 μg/ml.

Kit Components Only

Product No.
Description
  • Ammonia Assay Reagent 10 vial(s)
  • L-Glutamate Dehydrogenase for Ammonia Assay 1 mL/vial
  • Ammonia Standard Solution 1 mL/vial

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H319,H334

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1

Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

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Tingting Xiang et al.
Nature communications, 11(1), 108-108 (2020-01-09)
In cnidarian-Symbiodiniaceae symbioses, algal endosymbiont population control within the host is needed to sustain a symbiotic relationship. However, the molecular mechanisms that underlie such population control are unclear. Here we show that a cnidarian host uses nitrogen limitation as a
Junpeng Jiang et al.
BMC plant biology, 19(1), 239-239 (2019-06-07)
Ammonium transporters (AMTs), a family of proteins transporting ammonium salt and its analogues, have been studied in many aspects. Although numerous studies have found that ammonium affects the interaction between plants and pathogens, the role of AMTs remains largely unknown
Renal expression of the ammonia transporters, Rhbg and Rhcg, in response to chronic metabolic acidosis.
Seshadri, R.
American Journal of Physiology: Renal Physiology, 290, F397-F408 (2006)
Kahina Mellahi et al.
Bioprocess and biosystems engineering, 42(5), 711-725 (2019-01-24)
Mammalian-inducible expression systems are increasingly available and offer an attractive platform for the production of recombinant proteins. In this work, we have conducted process development for a cumate-inducible GS-CHO cell-line-expressing rituximab. To cope with the limitations encountered in batch when
Kahina Mellahi et al.
Journal of biotechnology, 298, 45-56 (2019-04-09)
In order to maximize cell growth and productivity for an inducible CHO cell line expressing rituximab, various fed-batch culture strategies were investigated. In each case, the performance was evaluated for cultures induced at moderate and high cell density conditions (4 × 106
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