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MAK120

Sigma-Aldrich

Urease Activity Assay Kit

sufficient for 100 colorimetric tests

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NACRES:
NA.84

usage

sufficient for 100 colorimetric tests

application(s)

environmental

detection method

colorimetric

relevant disease(s)

gastrointestinal diseases

storage temp.

2-8°C

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storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

usage

sufficient for 100 colorimetric tests

usage

sufficient for 200 colorimetric tests

usage

sufficient for 100 colorimetric tests (with TNB standard)

usage

sufficient for 100 colorimetric tests

application(s)

environmental

application(s)

-

application(s)

-

application(s)

-

relevant disease(s)

gastrointestinal diseases

relevant disease(s)

cancer; immunological diseases; gastrointestinal diseases

relevant disease(s)

cancer; gastrointestinal diseases; pulmonary disorders

relevant disease(s)

endocrinological disorders, diabetes; gastrointestinal diseases

General description

Urease is an enzyme that catalyzes the hydrolysis of urea into carbon dioxide and ammonia. Urease is found in bacteria, yeast, and certain higher plant species. Urease activity is found in environmental and fecal samples.

Application

Urease Activity Assay Kit has been used to measure urease activity.

Features and Benefits

Compatible with high-throughput handling systems. Can be adapted for use with cuvettes.

Suitability

Suitable for the detection of Urease activity in biological and environmental samples.

Principle

In this assay, urease catalyzes the hydrolysis of urea resulting in the production of ammonia. The ammonia is determined by the Berthelot method resulting in colorimetric product measure at 670 nm, proportionate to the urease activity present in the sample. One unit of urease is the amount of enzyme that catalyzes the formation of 1.0 μmole ammonia per minute at pH 7.0.

signalword

Danger

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Met. Corr. 1 - Muta. 2 - Skin Corr. 1B

supp_hazards

Storage Class

8B - Non-combustible, corrosive hazardous materials

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


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25G
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705578-5MG-PW

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MMYOMAG-74K-13

1000309185

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To limit nitrogen (N) losses from the soil, it has been suggested to provide urea to crops in conjunction with the urease inhibitor N-(n-butyl) thiophosphoric triamide (NBPT). However, recent studies reported that NBPT affects urea uptake and urease activity in

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