MilliporeSigma
All Photos(4)

Documents

U1500

Sigma-Aldrich

Urease from Canavalia ensiformis (Jack bean)

Type III, powder, 15,000-50,000 units/g solid

Sign Into View Organizational & Contract Pricing

Synonym(s):
Jack bean urease, Urea amidohydrolase
CAS Number:
Enzyme Commission number:
EC Number:
MDL number:
eCl@ss:
32160410

biological source

Canavalia ensiformis

type

Type III

form

powder

specific activity

15,000-50,000 units/g solid

mol wt

~544620 Da

application(s)

diagnostic assay manufacturing

storage temp.

2-8°C

Looking for similar products? Visit Product Comparison Guide

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
U0251U187594281
form

powder

form

powder

form

glycerol solution

form

powder

specific activity

15,000-50,000 units/g solid

specific activity

≥600,000 units/g solid

specific activity

-

specific activity

~8 U/mg

mol wt

~544620 Da

mol wt

~544620 Da

mol wt

~544620

mol wt

Mr ~480000

application(s)

diagnostic assay manufacturing

application(s)

-

application(s)

-

application(s)

-

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

General description

Subunit molecular weight: ~90,770
Composed of six subunits with total molecular weight: ~544,620
Urease is a cytosolic enzyme that catalyzes the hydrolysis of urea to ammonia and carbon dioxide, with initial conversion to carbamate, and the carbamate breaks down further, on hydrolysis, into ammonia and to carbonic acid / carbonate. Urease is a metalloenzyme that contains nickel as a co-factor. Urease occurs naturally in plants, fungi, and certain bacteria. James B. Sumner first isolated urease from jack bean in 1926.

Biochem/physiol Actions

Urease from Canavalia ensiformis functions as an insecticide by the release of a toxic small peptide cleaved by proteases found in the gut of insects.

Unit Definition

One micromolar unit will liberate 1.0 μmole of NH3 from urea per min at pH 7.0 at 25 °C. It is equivalent to 1.0 I.U. or 0.054 Sumner unit (1.0 mg ammonia nitrogen in 5 minutes at pH 7.0 at 20 °C)

Pictograms

Exclamation markHealth hazard

Signal Word

Danger

Hazard Statements

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

11 - Combustible Solids

WGK

WGK 1


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Customers Also Viewed

Slide 1 of 4

1 of 4

Jack beans fine powder

Sigma-Aldrich

J0125

Jack beans

Jack beans Whole Beans

Sigma-Aldrich

J0250

Jack beans

Sun-Il Kim et al.
Microorganisms, 10(1) (2022-01-22)
Ammonia from livestock manure reacts with chemical components discharged from various emission sources to produce airborne particulate matter. This study aimed to investigate a novel effective microbial agent to suppress ammonia gas emitted from manure. Both isolated L12I and 12III
Xiang-Zhen Yan et al.
Macromolecular bioscience, 14(7), 1004-1014 (2014-04-02)
The aim of this study is to optimize enzymatic control over gelation of chitosan-based hydrogels for the delivery of periodontal ligament cells (PDLCs). The results reveal that the gelation time, strength, and degradation rate of the chitosan hydrogels can be
Arlette Yumba-Mpanga et al.
Current medicinal chemistry, 26(1), 216-231 (2017-10-11)
In regard to urogenital tract cancer studies, an estimated 340,650 new cases and 58,360 deaths from genital system cancer and about 141,140 new cases and 29330 deaths from urinary system were projected to occur in the United States in 2012.
Jungyeon Kim et al.
PloS one, 15(3), e0230072-e0230072 (2020-03-18)
In the urinary metabolomics for finding biomarkers in urine, owing to high concentrations of urea, for chromatography-based metabolomic analysis, urea needed to be degraded by urease. This urease pretreatment has been the key step of sample preparation for standard urinary
Karl Norris et al.
Marine drugs, 18(6) (2020-06-06)
Whey protein isolate (WPI) is a by-product from the production of cheese and Greek yoghurt comprising β-lactoglobulin (β-lg) (75%). Hydrogels can be produced from WPI solutions through heating; hydrogels can be sterilized by autoclaving. WPI hydrogels have shown cytocompatibility and

Articles

Probiotics exhibit an inhibitory effect on pathogens, help prevent chronic intestinal inflammatory diseases or atopic syndromes, and support the immune system.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service