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27048

Millipore

Christensen′s Urea Agar

NutriSelect® Basic, suitable for microbiology

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Synonym(s):
Urea Agar Base acc. to Christensen, Urea Test Agar
NACRES:
NA.74

agency

according to GB 4789.4-2016
according to ISO 10273:2017
according to ISO 19250:2010
according to ISO 6579-1:2017

Quality Level

sterility

non-sterile

product line

BioChemika

form

powder

shelf life

limited shelf life, expiry date on the label

composition

agar, 12 g/L
gelatine peptone, 1 g/L
D(+)-glucose, 1 g/L
phenol red, 0.012 g/L
potassium dihydrogen phosphate, 2 g/L
sodium chloride, 5 g/L

manufacturer/tradename

NutriSelect® Basic

technique(s)

microbe id | specific enzyme detection: suitable
microbiological culture: suitable

final pH

6.8±0.2 (25 °C)

application(s)

clinical testing
environmental
food and beverages

microbiology

suitability

nonselective and differential for Enterobacter spp.
nonselective and differential for Escherichia coli
nonselective and differential for Klebsiella spp.
nonselective and differential for Proteus spp.
nonselective and differential for Yersinia spp.
nonselective and differential for coliforms

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Christensen′s Urea Agar

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Enterococcus Selective Agar

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Glucose Bromcresol Purple Agar

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Columbia Agar

technique(s)

microbe id | specific enzyme detection: suitable, microbiological culture: suitable

technique(s)

microbiological culture: suitable

technique(s)

microbe id | utilization test: suitable

technique(s)

microbe id | specific enzyme detection: suitable, microbiological culture: suitable

form

powder

form

powder

form

powder

form

powder

composition

agar, 12 g/L , D(+)-glucose, 1 g/L , potassium dihydrogen phosphate, 2 g/L , gelatine peptone, 1 g/L , sodium chloride, 5 g/L , phenol red, 0.012 g/L

composition

agar, 10 g/L , disodium hydrogen phosphate monohydrate, 4 g/L , D(+)-glucose, 2 g/L , sodium azide, 0.4 g/L , 2,3,5-triphenyltetrazolium chloride, 0.1 g/L , tryptose, 20 g/L , yeast extract, 5 g/L

composition

Agar, 15.0 g/L , Brom cresol purple, 0.015 g/L , Casein enzymic hydrolysate, 10.0 g/L , Glucose, 10.0 g/L , Sodium chloride, 5.0 g/L , Yeast extract, 1.5 g/L

composition

agar, 15.0 g/L , sodium chloride, 5.0 g/L , special nutrient substrate, 23.0 g/L , starch, 1.0 g/L

suitability

nonselective and differential for Enterobacter spp., nonselective and differential for Klebsiella spp., nonselective and differential for Yersinia spp., nonselective and differential for Escherichia coli, nonselective and differential for coliforms, nonselective and differential for Proteus spp.

suitability

selective and differential for Enterococcus spp., selective and differential for Streptococcus spp.

suitability

Citrobacter spp., Neisseria spp., Proteus spp., Serratia spp., Shigella spp., Streptococcus spp., nonselective and differential for Bacillus cereus, nonselective and differential for Bacillus spp., nonselective and differential for Enterobacter spp., nonselective and differential for Escherichia coli, nonselective and differential for Klebsiella spp., nonselective and differential for Salmonella spp., nonselective and differential for coliforms, nonselective and differential for enterobacteriaceae

suitability

Gardnerella spp., Haemophilus spp., Pneumococcus spp., nonselective for Bacteroides spp., nonselective for Campylobacter spp., nonselective for Clostridium spp., nonselective for Corynebacterium spp., nonselective for Enterococcus spp., nonselective for Escherichia coli, nonselective for Neisseria spp., nonselective for Staphylococcus spp., nonselective for Streptococcus spp., nonselective for bacteria (General Media), nonselective for coliforms

sterility

non-sterile

sterility

non-sterile

sterility

non-sterile

sterility

non-sterile

Application

For the differentiation of urea-metabolizing microorganisms. Weak urease producers such as klebsiella, enterobacter etc. can also be differentiated. Yersinia pseudotuberculosis (urea positive) from Yersinia pestis (urea negative) acc. to Thal and Chen (1955).

Preparation Note

Dissolve 21 g in 950 ml distilled water. Sterilize by autoclaving at 121°C for 15 minutes. After cooling to 50°C, add aseptically 50ml (10 vials) sterile filtered 40% urea solution (Cat. No. 08582) and let cool as slants.

Other Notes

Screening of enteric pathogens from stool specimens

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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Customers Also Viewed

Slide 1 of 7

1 of 7

W.B. Christensen
Journal of Bacteriology, 69, 103-103 (1946)
P A Villasante et al.
Journal of clinical microbiology, 25(3), 584-585 (1987-03-01)
A total of 800 colonies suggestive of Salmonella, Shigella, or Yersinia species isolated on stool differential agar media were inoculated onto both conventional biochemical test media (triple sugar iron agar, urea agar, and phenylalanine agar) and Entero Pathogen Screen cards
Two simple tests for the differentiation of plague and pseudo tuberculosis bacilli.
E THAL et al.
Journal of bacteriology, 69(1), 103-104 (1955-01-01)

Articles

An article concerning selective growth media for differentiation and detection of Escherichia coli and other coliforms.

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