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GranuCult® Triple sugar iron agar

pkg of 500 g, for Enterobacter spp., for Salmonella spp., for Shigella spp., degradation of sugar and accompanying acid production is detected by the pH indicator phenol red

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TSI agar

Quality Level


medium granules (dehydrated (DCM))


pkg of 500 g




microbe id | utilization test: suitable
microbiological culture: suitable


7.4 (37 °C, 65 g/L in H2O, after autoclaving)


65 g/L

bulk density

650 kg/m3


food and beverages

storage temp.



Citrobacter spp.
Enterobacter spp.
Escherichia coli
Klebsiella spp.
Proteus spp.
Salmonella spp.
Shigella spp.

General description

GranuCult® Triple sugar iron agar is recommended for the identification of Gram-negative enterobacteria. It contains lactose, sucrose, and glucose as fermentable carbohydrates. The pH indicator, phenol red, indicates the degradation of sugars to produce acids by the varying color of the medium (from red to yellow). Several species of bacteria reduce thiosulfate to hydrogen sulfide that combines with ferric ions of iron salt to produce iron sulfide, an insoluble black precipitate.


GranuCult® Triple sugar iron agar is suitable for the identification of Enterobacteriaceae in meat, meat products, and milk. It is commonly used as a biochemical test in characterization of a microorganism. Depending on the extent of fermentation and production of gas or production of H2S, the type of the microorganism can be determined.

Features and Benefits

  • Safe and sustainable due to reduced risks associated with fine dust and toxic substance inhalation, resulting in a safer work environment
  • Excellent wettability, solubility, and free flowing properties
  • Convenient, with minimal component separation and clumping, even under warm or humid conditions
  • High batch-to- batch reproducibility
  • Prolonged shelf life of up to five years
  • High number of test strains exceeding all regulatory demands
  • Granulation technology allowing many supplements to be included, with no need to add these separately

Analysis Note

Appearance (clearness): clear
Appearance (colour): red
pH-value (25 °C): 7.2 - 7.6
Growth (Escherichia coli ATCC 25922 (WDCM 00013)): good to very good
Growth (Citrobacter freundii ATCC 8090): good to very good
Growth (Enterobacter cloacae ATCC 13047 (WDCM 00083)): good to very good
Growth (Shigella flexneri ATCC 12022 (WDCM 00126)): good to very good
Growth (Salmonella typhimurium ATCC 14028 (WDCM 00031)): good to very good
Growth (Salmonella enteritidis ATCC 13076 (WDCM 00030)): good to very good
Growth (Proteus mirabilis ATCC 14153): good to very good
Growth (Proteus hauseri ATCC 13315): good to very good
Butt (Escherichia coli ATCC 25922 (WDCM 00013)): yellow
Butt (Citrobacter freundii ATCC 8090): yellow/black
Butt (Enterobacter cloacae ATCC 13047 (WDCM 00083)): yellow
Butt (Shigella flexneri ATCC 12022 (WDCM 00126)): yellow
Butt (Salmonella typhimurium ATCC 14028 (WDCM 00031)): yellow/black
Butt (Salmonella enteritidis ATCC 13076 (WDCM 00030)): yellow/black
Butt (Proteus mirabilis ATCC 14153): yellow/black
Butt (Proteus hauseri ATCC 13315): yellow/black
Slant surface (Escherichia coli ATCC 25922 (WDCM 00013)): yellow
Slant surface (Citrobacter freundii ATCC 8090): yellow
Slant surface (Enterobacter cloacae ATCC 13047 (WDCM 00083)): yellow
Slant surface (Shigella flexneri ATCC 12022 (WDCM 00126)): red
Slant surface (Salmonella typhimurium ATCC 14028 (WDCM 00031)): red
Slant surface (Salmonella enteritidis ATCC 13076 (WDCM 00030)): red
Slant surface (Proteus mirabilis ATCC 14153): red/black
Slant surface (Proteus hauseri ATCC 13315): yellow
Incubation: 24 hrs.; 35 °C; aerobic.


We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids



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