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85463

Millipore

Simmons Citrate Agar

suitable for microbiology, NutriSelect® Basic

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Synonym(s):
Citrate Utilization Test
NACRES:
NA.74

sterility

non-sterile

Quality Level

product line

BioChemika

form

powder

shelf life

limited shelf life, expiry date on the label

composition

agar, 15 g/L
ammonium dihydrogen phosphate, 0.2 g/L
bromothymol blue, 0.08 g/L
disodium ammonium phosphate, 0.8 g/L
magnesium sulfate heptahydrate, 0.2 g/L
sodium chloride, 5 g/L
trisodium citrate, 2 g/L

manufacturer/tradename

NutriSelect® Basic

technique(s)

microbe id | utilization test: suitable
microbiological culture: suitable

final pH

~7.0

application(s)

agriculture
clinical testing
environmental
food and beverages
water monitoring

microbiology

suitability

nonselective and differential for Escherichia coli
nonselective and differential for Salmonella spp.
nonselective and differential for coliforms
nonselective and differential for molds (General Media)
nonselective and differential for yeasts (General Media)

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Simmons Citrate Agar suitable for microbiology, NutriSelect® Basic

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form

powder

form

powder

form

powder

form

powder

shelf life

limited shelf life, expiry date on the label

shelf life

limited shelf life, expiry date on the label

shelf life

limited shelf life, expiry date on the label

shelf life

limited shelf life, expiry date on the label

composition

agar, 15 g/L , ammonium dihydrogen phosphate, 0.2 g/L , bromothymol blue, 0.08 g/L , disodium ammonium phosphate, 0.8 g/L , magnesium sulfate heptahydrate, 0.2 g/L , sodium chloride, 5 g/L , trisodium citrate, 2 g/L

composition

Agar, 15.0 g/L , Brom cresol purple, 0.015 g/L , Casein enzymic hydrolysate, 10.0 g/L , Glucose, 10.0 g/L , Sodium chloride, 5.0 g/L , Yeast extract, 1.5 g/L

composition

agar, 15 g/L , beef extract, 3 g/L , bromo thymol blue, 0.02 g/L , casein enzymic hydrolysate, 4 g/L , L-cystine, 0.128 g/L , lactose, 10 g/L , peptic digest of animal tissue, 4 g/L

composition

agar, 12 g/L , chromogenic mixture, 0.2 g/L , dipotassium hydrogen phosphate, 3 g/L , peptone, special, 3 g/L , potassium dihydrogen phosphate, 1.7 g/L , sodium chloride, 5 g/L , sodium lauryl sulfate, 0.1 g/L , sodium pyruvate, 1 g/L , tryptophan, 1 g/L

manufacturer/tradename

NutriSelect® Basic

manufacturer/tradename

NutriSelect® Plus

manufacturer/tradename

NutriSelect® Plus

manufacturer/tradename

NutriSelect® Plus

technique(s)

microbe id | utilization test: suitable, microbiological culture: suitable

technique(s)

microbe id | utilization test: suitable

technique(s)

microbe id | utilization test: suitable, microbiological culture: suitable

technique(s)

-

General description

Simmons Citrate Agar uses citric acid as the carbon source. It is used to differentiate Salmonella groups from each other. It does not allow the growth of Shigella or coliforms.

Application

Simmons Citrate Agar can be used for differentiating between fecal coli and members of the aerogenes group based on citrate utilization. It can also be used for the identification of certain fungi and fungi imperfecti. The agar has been used to perform a citrate test.

Preparation Note

Suspend 23.3 g in 1 litre distilled water and heat to boiling to dissolve. Distribute in tubes or flasks and sterilize by autoclaving at 121°C for 15 minutes. Allow tubes to cool as slants.

Other Notes

Selective recovery of Klebsiella pneumoniae and Klebsiella oxytoca from human feces

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Articles

Salmonella contamination is the second leading cause of food-borne illness worldwide. Controlling outbreaks of Salmonella is an important task for food regulators, restaurants and the food industry in general. The Salmonella family includes over 2,300 serotypes of bacteria, but two types, Salmonella enteritidis and Salmonella typhimurium, are responsible for about half of all human infections. Most outbreaks of Salmonella are traced back to dairy, poultry and meat products, but Salmonella can grow on nearly any food. Chicken, eggs and their derivative products are particularly high risk.

An article concerning selective growth media for differentiation and detection of Escherichia coli and other coliforms.

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