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Lysine Decarboxylase Broth

NutriSelect® Plus, suitable for microbiology

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Synonym(s):
LD Broth
NACRES:
NA.85

agency

according to ISO 10273:2017

Quality Level

sterility

non-sterile

form

powder

shelf life

limited shelf life, expiry date on the label

composition

bromo creol purple, 0.02 g/L
dextrose, 1 g/L
L-lysine hydrochloride, 5 g/L
peptic digest of animal tissue, 5 g/L
yeast extract, 3 g/L

manufacturer/tradename

NutriSelect® Plus

technique(s)

microbe id | specific enzyme detection: suitable
microbiological culture: suitable

final pH

6.8±0.2 (25 °C)

application(s)

clinical testing
environmental
food and beverages

microbiology

suitability

Enterobacter spp.
nonselective and differential for Salmonella spp.
nonselective and differential for enterobacteriaceae

Application

Lysine Decarboxylase Broth is used for distinguishing the Salmonella arizonae from the Bethesda Bellerup Group of Enterobacteriaceae.

Preparation Note

Suspend 14g in 1000ml distilled water. Boil to dissolve the medium completely. Dispense in 5 ml amount into screw capped test tubes. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool the tubed medium in an upright position. Inoculate the tubes and overlay with 2-3 ml of sterile mineral oil.

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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