MAK538

Ammonia Assay Kit

sufficient for 100 colorimetric or fluorometric tests

• UNSPSC Code: 12161503
• NACRES: NA.84

Properties

• Quality Level: 100
• detection method: colorimetric; fluorometric
• relevant disease(s): gastrointestinal diseases
• shipped in: wet ice
• storage temp.: −20°C

Description

General description

Ammonia (NH3) or its ion form ammonium (NH4 +) is an important source of nitrogen for living systems. It is synthesized through amino acid metabolism and is toxic when present at high concentrations. In the liver, ammonia is converted to urea through the urea cycle. Elevated levels of ammonia in the blood (hyperammonemia) have been found in liver dysfunction (cirrhosis), while hypoammonemia has been associated with defects in the urea cycle enzymes (e.g. ornithine transcarbamylase).

Features and Benefits

• Linear detection range: 24 to 1000 µM

Suitability

The kit is suitable for ammonia determination in biological samples (e.g. serum, plasma, urine, saliva, cell culture, etc).

Principle

The Ammonia Assay Kit is designed to directly measure NH₃ and NH4 +. In this assay, NADH is converted to NAD⁺ in the presence of NH₃, ketoglutarate and glutamate dehydrogenase. The decrease in optical density at 340 nm or fluorescence intensity at λEx = 360 nm/λEm = 450 nm is directly proportionate to the NH3 concentration in the sample.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 3