252549

Formaldehyde solution

ACS reagent, 37 wt. % in H₂O, contains 10-15% Methanol as stabilizer (to prevent polymerization)

• Synonym(s): Formalin
• Linear Formula: HCHO
• CAS Number: 50-00-0
• Molecular Weight: 30.03
• Beilstein/REAXYS Number: 1209228
• MDL number: MFCD00003274
• UNSPSC Code: 12352114
• PubChem Substance ID: 24855218
• NACRES: NA.21

Properties

• grade: ACS reagent
• Quality Level: 200
• vapor density: 1.03 (vs air)
• vapor pressure: 52 mmHg ( 37 °C); 52 mmHg ( 37 °C)
• form: liquid
• autoignition temp.: 572 °F
• contains: 10-15% Methanol as stabilizer (to prevent polymerization)
• concentration: 36.5-38.0%; 37 wt. % in H₂O
• impurities: ≤0.006 meq/g Titr. acid
• ign. residue: ≤0.005%
• color: APHA: ≤10
• refractive index: n20/D 1.3765
• density: 1.09 g/mL at 25 °C (lit.)
• anion traces: chloride (Cl^-): ≤5 ppm; sulfate (SO₄^2-): ≤0.002%
• cation traces: Fe: ≤5 ppm; heavy metals: ≤5 ppm (by ICP-OES)
• suitability: suitable for manufacturing use (Laboratory chemicals)
• SMILES string: [H]C([H])=O
• InChI: 1S/CH2O/c1-2/h1H2
• InChI key: WSFSSNUMVMOOMR-UHFFFAOYSA-N

Description

General description

Formaldehyde (formalin) is produced by oxidation of methanol. It is made of 37% formaldehyde and impurities such as methanol, small amounts of formic acid, aldehydes and ketones. It is used as a denaturant in formaldehyde-agarose gel electrophoresis of RNA.

Application

Formaldehyde solution has been used as a fixing agent to fix cells during immunofluorescence imaging and for cross-linking cells during chromatin immunoprecipitation (ChIP) assay.

Formaldehyde solution has been used for cross-linking/fixing of cells in ChIP (chromatin immunoprecipitation) assay. It has been used for fixing of cells for imaging.

Physical form

This product is a solution of approximately 37% by weight of formaldehyde gas in water.

Safety Information

• pictograms: GHS02,GHS06,GHS08,GHS05
• signalword: Danger
• hcodes: H226,H301 + H311,H314,H317,H330,H335,H341,H350,H370
• pcodes: P202 - P210 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 2 Inhalation - Acute Tox. 3 Dermal - Acute Tox. 3 Oral - Carc. 1B - Eye Dam. 1 - Flam. Liq. 3 - Muta. 2 - Skin Corr. 1B - Skin Sens. 1 - STOT SE 1 - STOT SE 3
• target_organs: Eyes,Central nervous system, Respiratory system
• Storage Class: 3 - Flammable liquids
• wgk_germany: WGK 3
• flash_point_f: 132.8 °F - closed cup
• flash_point_c: 56 °C - closed cup