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CS0170

Sigma-Aldrich

Thioredoxin Reductase Assay Kit

1 kit sufficient for 100 assays (1 mL)

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NACRES:
NA.84

Quality Level

usage

 kit sufficient for 100 assays (1 mL)

shipped in

dry ice

storage temp.

−20°C

Gene Information

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CS0170

Thioredoxin Reductase Assay Kit

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Gene Information

human ... TXNRD1(7296), TXNRD2(10587), TXNRD3(114112)

Gene Information

human ... CS(1431)

Gene Information

human ... CASP3(836)

Gene Information

human ... TXNRD2(10587)

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

usage

 kit sufficient for 100 assays (1 mL)

usage

 kit sufficient for 100 reactions (using a 1 ml cuvette),  kit sufficient for 480 reactions (using 96 multiwell plates)

usage

sufficient for 1,000 multiwell tests (in 96-well multiwell plates), sufficient for 100 standard tests (1 ml)

usage

-

General description

The Thioredoxin Reductase Assay Kit uses a colorimetric assay for the determination of thioredoxin reductase activity in mammalian cells and tissues. It is based on the reduction of 5,5′-dithiobis(2-nitrobenzoic) acid (DTNB) with NADPH to 5-thio-2-nitrobenzoic acid (TNB) that produces a strong yellow color that is measured at 412 nm.

DTNB + NADPH + H+ ↔ 2 TNB + NADP+

Application

Using post nuclear extracts of rat liver, the kit shows linearity up to 0.25 units per mL. In this preparation, ~20% of the activity is due to non-specific reductase activity (refractory to the TrxR inhibitor).

Features and Benefits

  • An easy and simple method
  • The kit contains all the reagents required for a colorimetric assay of mammalian thioredoxin reductase
  • The kit includes an inhibitor solution for specific inhibition of mammalian thioredoxin reductase
  • The kit has been tested with heart muscle, as well as lysates from cell lines such as HeLa, A549, Jurkat, U937, A431, COS, CHO, and NIH 3T3 cells

Kit Components Only

Product No.
Description

  • Assay Buffer 5x for Thioredoxin Reductase 30 mL

  • Thioredoxin Reductase 10 μg

  • Thioredoxin Reductase Inhibitor Solution .05 mL

  • 5,5'-Dithiobis(2-nitrobenzoic) acid (DNTB) 150 mg

  • NADPH 25 mg

  • Dimethyl Sulfoxide (DMSO) 7.5 mL

pictograms

Health hazard

signalword

Warning

hcodes

Hazard Classifications

Repr. 2

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

188.6 °F

flash_point_c

87 °C


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