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Rat/Mouse Insulin ELISA

This Rat/Mouse Insulin ELISA is used to measure & quantify Insulin levels in Metabolism & Endocrine research.

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Synonym(s):
Insulin
eCl@ss:
32161000
NACRES:
NA.32

Quality Level

species reactivity

mouse, rat

assay range

accuracy: 83-102%
linearity: 79-133%
sensitivity: 0.2 ng/mL
standard curve range: 0.2-10 ng/mL

technique(s)

ELISA: suitable

UniProt accession no.

application(s)

research use

detection method

colorimetric
fluorometric

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Rat/Mouse Insulin ELISA This Rat/Mouse Insulin ELISA is used to measure & quantify Insulin levels in Metabolism & Endocrine research.

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Rat/Mouse C-Peptide 2 ELISA This Rat/Mouse C-Peptide 2 ELISA is used to measure & quantify C-Peptide 2 levels in Metabolism & Endocrine & Neuroscience research.

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assay range

accuracy: 83-102%, sensitivity: 0.2 ng/mL, linearity: 79-133%, standard curve range: 0.2-10 ng/mL

assay range

accuracy: 76-103%, linearity: 96-112%, standard curve range: 2-200 μU/mL

assay range

accuracy: 88-128%, linearity: 73-100%, sensitivity: 0.73 μU/mL, standard curve range: 2-200 μU/mL

assay range

accuracy: 86-87%
(Mouse Serum), accuracy: 88-92%
(Rat Serum), accuracy: 93-95%
(Rat Plasma), linearity: 86-108%, standard curve range: 25-1600 pM

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

technique(s)

ELISA: suitable

technique(s)

ELISA: suitable

technique(s)

ELISA: suitable

technique(s)

ELISA: suitable

detection method

colorimetric, fluorometric

detection method

colorimetric, fluorometric

detection method

colorimetric, fluorometric

detection method

colorimetric, fluorometric

UniProt accession no.

P01322, P01325

UniProt accession no.

P01308

UniProt accession no.

P01308

UniProt accession no.

-

General description

Insulin is secreted by the islet β cells in the pancreas. It contains two peptide chains linked by disulfide bridges. The A chain is composed of 21 amino acids and the B chain has 30 amino acids.

Application

Rat/Mouse Insulin ELISA is suitable for measuring and quantifying insulin levels in metabolism and endocrine research. It has been used to determine plasma insulin by enzyme-linked immunosorbent assay. It has also been used to measure the insulin secretion from cells, in vitro.

Biochem/physiol Actions

Insulin is a key hormone that maintains normal blood glucose levels. It mediates cellular glucose uptake, controls carbohydrate, lipid, and protein metabolism, and enhances cell division and growth through its mitogenic effects. Insulin resistance is a condition of impaired sensitivity to insulin-mediated glucose disposal.

Other Notes

3 hour assay, room temperature 10 μL sample size
Research Category: Metabolism
Research Sub Category: Obesity/Metabolic Disorders

Disclaimer

For research use only. Not for use in diagnostic procedures.

pictograms

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Warning

Hazard Classifications

Met. Corr. 1 - Skin Sens. 1

Storage Class

8A - Combustible, corrosive hazardous materials

wgk_germany

WGK 3


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