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RAB0229

Sigma-Aldrich

Mouse IGF-I ELISA Kit

for serum, plasma and cell culture supernatant

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Synonym(s):
Insulin-like growth factor I, Mechano growth factor, Somatomedin-C
UNSPSC Code:
41116158
NACRES:
NA.32

species reactivity

mouse

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable
capture ELISA: suitable

input

sample type cell culture supernatant(s)
sample type serum
sample type plasma

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 4 pg/mL
standard curve range: 2.74-2000 pg/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

mouse ... Igf1(16000)

General description

The Mouse IGF-I ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse IGF-I in serum, plasma, cell culture supernatants and urine.

Immunogen

Recombinant Mouse IGF-I

Application

For research use only. Not for use in diagnostic procedures.
Mouse IGF-I ELISA kit has been used to measure plasma IGF-I (Insulin-like growth factor-I) level from Igf1r L/L and BAT-specific Igf1r-knockout (BATIGFIRKO) mice.
For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)
Mouse IGF-I ELISA Kit has been used to measure the concentration of insulin-like growth factor(IGF) secreted by mesenchymal stem cells(MSCs). It has also been used to measure the level of IGF1 in blood samples of mice.

Biochem/physiol Actions

IGF-I (Insulin-like growth factor-I) is required for fetal growth and development. It is also involved in the growth and repair of heart tissues. IGF-I has been linked to neuroplasticity and hippocampal neurogenesis. It is important in the recovery from ischaemic injury. IGF-I also facilitates the differentiation of adipocytes and other cells.

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit Components Also Available Separately

Product No.
Description
SDS

  • RABELADAELISA 1X Assay/Sample Diluent Buffer A (Item D1)SDS

  • RABELADBELISA 5X Assay/Sample Diluent Buffer B (Item E1)SDS

  • RABSTOP3ELISA Stop Solution (Item I)SDS

  • RABTMB3ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)SDS

  • RABWASH420X Wash Buffer (Item B)SDS

pictograms

Corrosion

signalword

Warning

hcodes

Hazard Classifications

Met. Corr. 1

wgk_germany

WGK 3


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Flavia R Siqueira et al.
Physiology & behavior, 154, 68-75 (2015-11-26)
A low-salt (LS) diet during pregnancy has been linked to insulin resistance in adult offspring, at least in the experimental setting. However, it remains unclear if this effect is due to salt restriction during early or late pregnancy. To better
He He et al.
Inflammation, 38(6), 2178-2184 (2015-06-25)
Sepsis-induced myocardial injury (SIMI) is caused by various mechanisms. The aim of this study was to investigate the effects of salidroside (Sal) on SIMI and its mechanisms in rats. The sepsis model was established by intraperitoneal injection of lipopolysaccharide (LPS)
Sebastian Mathes et al.
Journal of molecular medicine (Berlin, Germany), 100(11), 1647-1658 (2022-10-01)
Skeletal muscle exerts many beneficial effects on the human body including the contraction-dependent secretion of peptides termed myokines. We have recently connected the myokine secreted protein acidic and rich in cysteine (SPARC) to the formation of intramuscular adipose tissue (IMAT)
María Llorens-Martín et al.
The Neuroscientist : a review journal bringing neurobiology, neurology and psychiatry, 15(2), 134-148 (2009-03-25)
This review addresses the role of serum insulin-like growth factor 1 (IGF1) as one mechanism of adult neural plasticity, specifically, its regulation of hippocampal neurogenesis among other plasticity-related processes. It is suggested that IGF has been reused advantageously both for
Márcio Buffolo et al.
Cell reports, 29(2), 270-282 (2019-10-10)
Accumulation of visceral (VIS) is a predictor of metabolic disorders and insulin resistance. This is due in part to the limited capacity of VIS fat to buffer lipids allowing them to deposit in insulin-sensitive tissues. Mechanisms underlying selective hypertrophic growth

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