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EZGLP1T

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Multi Species GLP-1 Total ELISA, EZGLP1T-36K

This Multi Species GLP-1 Total ELISA, EZGLP1T-36K, is used to measure & quantify Glucagon Like Peptide-1 levels in Metabolism research.

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Synonym(s):
Glucagon-like peptide-1
eCl@ss:
32161000
NACRES:
NA.32

Quality Level

species reactivity

human, mouse, rat

assay range

linearity: 90-132%
recovery range: 90-110%
sensitivity: 1.5 pM
(MinDC)

standard curve range: 4.1-1000 pM
inter-assay cv: <12%
intra-assay cv: <5%

technique(s)

ELISA: suitable

NCBI accession no.

UniProt accession no.

application(s)

research use

Gene Information

human ... GCG(2641)

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RAB0671EZGLPHSEZRMGIP
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RAB0671

Human GCG / Glucagon ELISA Kit

Rat/Mouse GIP (total) ELISA This Rat/Mouse GIP (total) ELISA is used to measure &amp; quantify GIP levels in Metabolism &amp; Endocrine research.

EZRMGIP

Rat/Mouse GIP (total) ELISA

species reactivity

human, mouse, rat

species reactivity

human

species reactivity

mouse, rat, human

species reactivity

mouse, rat

assay range

linearity: 90-132%, sensitivity: 1.5 pM
(MinDC), inter-assay cv: <12%
intra-assay cv: <5%, recovery range: 90-110%, standard curve range: 4.1-1000 pM

assay range

-

assay range

-

assay range

-

Quality Level

200

Quality Level

-

Quality Level

200

Quality Level

200

technique(s)

ELISA: suitable

technique(s)

ELISA: suitable

technique(s)

ELISA: suitable

technique(s)

ELISA: suitable

NCBI accession no.

NM_002054.2

NCBI accession no.

-

NCBI accession no.

-

NCBI accession no.

-

General description

Glucagon-like peptide-1 (GLP-1) is a 30 amino acid gut hormone secreted from the intestinal L-cells. The GLP-1 sequence is highly conserved among mammals and is released in its active form (7-36) in response to food intake. It is quickly degraded into its inactive form (9-36) after cleavage by DPPIV. GLP-1 reduces glucose levels by regulating pancreatic secretion, slowing gastric emptying and lowering the desire for food intake. Therefore, it is an important biomarker for the study of diabetes and obesity. This kit measures both the active (7-36) and inactive (9-36) forms of GLP-1.

Specificity

Cross Reactivty
GLP-1 is conserved in almost all mammals.
The antibody pair used in this assay is specific to GLP-1 (7-36) and (9-36) and has no cross-reactivity with GLP-2, GIP, Glucagon or Oxyntomodulin.

Application

Research Category
Metabolism
This is a 3.5-hour assay at room temperature requiring 50 uL of serum, plasma or culture samples per well.
Used to detect/quantify: Glucagon Like Peptide-1

Disclaimer

For research use only. Not for use in diagnostic procedures.

signalword

Danger

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Met. Corr. 1 - Skin Sens. 1

Storage Class

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk_germany

WGK 3


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Lindsey M Rings et al.
Journal of veterinary internal medicine, 33(6), 2752-2764 (2019-10-31)
The enteroinsular axis (EIA) comprises intestinal factors (incretins) that stimulate insulin release after PO ingestion of nutrients. Glucose-dependent insulinotropic polypeptide (GIP) and glucagon-like peptide-1 (GLP-1) are the main incretins. The EIA has not been investigated in healthy neonatal foals but
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Physiological reports, 8(11), e14469-e14469 (2020-05-31)
The short-form glucose-dependent insulinotropic polypeptide (GIP) (1-30) is released from islet alpha cells and promotes insulin secretion in a paracrine manner in vitro. However, it is not well elucidated how GIP (1-30) is involved in glucose metabolism in vivo, since

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