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11191

Sigma-Aldrich

Histopaque®-1119

sterile-filtered, density: 1.119 g/mL

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NACRES:
NA.75

sterility

sterile-filtered

Quality Level

shelf life

Expiry date on the label.

impurities

endotoxin, tested

density

1.119 g/mL

application(s)

hematology
histology

storage temp.

2-8°C

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This Item
1077110831A6929
Histopaque®-1119 sterile-filtered, density: 1.119 g/mL

Sigma-Aldrich

11191

Histopaque®-1119

Histopaque®-1077 sterile-filtered, density: 1.077 g/mL

Sigma-Aldrich

10771

Histopaque®-1077

Sigma-Aldrich

Sigma-Aldrich

10831

Histopaque®-1083

shelf life

Expiry date on the label.

shelf life

Expiry date on the label.

shelf life

Expiry date on the label.

shelf life

Expiry date on the label.

density

1.119 g/mL

density

1.077 g/mL

density

1.083 g/mL

density

-

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

Quality Level

400

Quality Level

400

Quality Level

400

Quality Level

400

impurities

endotoxin, tested

impurities

endotoxin, tested

impurities

endotoxin, tested

impurities

-

General description

Histopaque®-1119 is a sterile, endotoxin tested, ready-to-use medium. This solution consists of polysucrose and sodium diatrizoate, adjusted to a density of 1.119 g/mL and pH 8.8–9.0.

Application

Histopaque®-1119 is suitable for use in conjunction with Histopaque-1077 as it facilitates the rapid recovery of viable mononuclear cells and granulocytes from small volumes of whole blood or bone marrow

Features and Benefits

Histopaque®-1119 offers numerous advantages, such as:
  • Optimal recovery of viable cells
  • Permits selective separation of blood cell types
  • Prevents cell distortion
  • Preserves cell viability
  • Minimal extraneous cell interference
  • Reproducible performance from batch to batch
  • Sterile-filtered and endotoxin-tested
  • Stable at 2-8°C for at least 2 years when protected from light

Principle

The process involves the formation of a double gradient on layering an equal volume of Histopaque-1077 over Histopaque-1119. Anticoagulated whole blood is carefully layered onto the upper Histopaque-1077 medium. Upon centrifugation, the erythrocytes are aggregated by polysucrose and thus rapidly sediment. Granulocytes are found at the lower Histopaque-1077/1119 interface; whereas, lymphocytes and other mononuclear cells are found at the upper plasma/Histopaque-1077 interface. Most extraneous platelets found in the upper interface are removed by low-speed centrifugation during washing steps. Erythrocyte contamination is negligible.

Legal Information

Histopaque is a registered trademark of Merck KGaA, Darmstadt, Germany

related product

Product No.
Description
Pricing

Pictograms

Corrosion

Signal Word

Warning

Hazard Statements

Hazard Classifications

Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2

Storage Class Code

8A - Combustible, corrosive hazardous materials

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Protocols

This troubleshooting guide addresses the most common sources of error observed when using Histopaque but is not meant to be a comprehensive list.

Ficoll 400® is a highly branched polymer formed by the copolymerization of sucrose and epichlorohydrin. Ficoll 400® is completely non-ionic. Because of the abundance of hydroxyl groups, Ficoll 400® is very hydrophilic and extremely water-soluble.

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