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H8889

Sigma-Aldrich

Histopaque®-1077 Hybri-Max™

liquid, sterile-filtered, BioReagent, suitable for hybridoma

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NACRES:
NA.75

sterility

sterile-filtered

Quality Level

400

product line

BioReagent

form

liquid

technique(s)

cell culture | hybridoma: suitable
single cell analysis: suitable

impurities

endotoxin, tested

density

1.077 g/cm3 at 25 °C (lit.)

storage temp.

2-8°C

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This Item
A69291077110831
Sigma-Aldrich

Sigma-Aldrich

H8889

Histopaque®-1077 Hybri-Max™

Sigma-Aldrich

Sigma-Aldrich

A6929

ACCUSPIN™ System-Histopaque®-1077

Histopaque®-1077 sterile-filtered, density: 1.077 g/mL

Sigma-Aldrich

10771

Histopaque®-1077

Sigma-Aldrich

Sigma-Aldrich

10831

Histopaque®-1083

form

liquid

form

solution

form

-

form

-

technique(s)

cell culture | hybridoma: suitable, single cell analysis: suitable

technique(s)

-

technique(s)

-

technique(s)

-

density

1.077 g/cm3 at 25 °C (lit.)

density

-

density

1.077 g/mL

density

1.083 g/mL

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

product line

BioReagent

product line

-

product line

-

product line

-

General description

Histopaque®-1077 is a solution comprising polysucrose and sodium diatrizoate, adjusted to a density of 1.077 +/- 0.001 g/cm3. This medium facilitates rapid recovery of viable lymphocytes from small volumes of whole blood. This technique is suitable for use in cell-mediated lympholysis and human lymphocyte antigen (HLA)2 typing. It may also be employed as the initial isolation step before the enumeration of T-, B-, and null lymphocytes. It is used to create a density medium for the purification of lymphocytes and other mononuclear cells.

Application

Histopaque®-1077 Hybri-Max™ has been used to isolate:
  • peripheral blood mononuclear cells (PBMCs) from heparinized blood by gradient centrifugation
  • immune cells (lymphocytes) from peripheral blood
  • PBMCs from blood

Legal Information

Histopaque is a registered trademark of Sigma-Aldrich Co. LLC
Hybri-Max is a trademark of Sigma-Aldrich Co. LLC

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H317 - H334

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

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25G
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1000309185

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Lymphoblastoid cell lines (LCLs) are generated by transforming primary B cells with Epstein-Barr virus (EBV) and are used extensively as model systems in viral oncology, immunology, and human genetics research. In this study, we characterized single-cell transcriptomic profiles of five
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Protocols

Histopaque® Density Gradient Media Troubleshooting Guide

This troubleshooting guide addresses the most common sources of error observed when using Histopaque but is not meant to be a comprehensive list.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service