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Merck
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문서

MAK331

Sigma-Aldrich

AF HDL and LDL/VLDL Assay Kit

sufficient for 100 colorimetric or fluorometric tests

동의어(들):

Total Cholesterol Assay Kit

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About This Item

UNSPSC 코드:
12161503
NACRES:
NA.84

사용

sufficient for 100 colorimetric or fluorometric tests

입력

plasma
tissue extract(s)
serum
food(s)

응용 분야

pharmaceutical

검출 방법

colorimetric

관련 질환(들)

cardiovascular diseases

저장 온도

−20°C

일반 설명

Cholesterol concentrations in High-Density Lipoprotein (HDL) and Low-Density (LDL)/Very-Low-Density (VLDL) Lipoproteins are strong predictors for coronary heart disease. Functional HDL offers protection by removing cholesterol from cells and atheroma. Higher concentrations of LDL and lower concentrations of functional HDL are strongly associated with cardiovascular disease due to higher risk of atherosclerosis. The balances between high- and low-density lipoproteins are solely genetically determined, but can be changed by medications, food choices and other factors. Simple, direct and automation-ready procedures for measuring HDL and LDL/VLDL concentrations are very desirable.

애플리케이션

The AF HDL and LDL/VLDL Assay Kit may be used for:
  • Cancer Research
  • Cardiovascular Research
  • Neurological Research

특징 및 장점

Efficient and Precise: Detect Cholesterol levels ranging from 1 to 100 mg/dL (colorimetric) and 0.2 to 10 mg/dL (fluorimetric) in a 30 minute room temperature reaction, providing both convenience and sensitivity.

Compatibility with High-Throughput Systems: Easily incorporate our kit into high-throughput handling systems, ensuring smooth and accurate processing, enhancing efficiency in your laboratory workflow.

적합성

Suitable for HDL and LDL/VLDL cholesterol detection in serum samples and for the evaluation of drugs on cholesterol metabolism.

원리

The AF HDL and LDL/VLDL Assay Kit is based on an improved PEG precipitation method in which HDL and LDL/VLDL are separated, and cholesterol concentrations are determined using a single Working Reagent that combines cholesterol ester hydrolysis, oxidation and color reaction in one step. The color intensity of the reaction product at 570nm or fluorescence intensity at λem/ex = 585/530nm is directly proportional to total cholesterol concentration in the sample. Linear detection range in 96-well plate: 1 to 100 mg/dL cholesterol for colorimetric assays and 0.2 to 10 mg/dL for fluorometric assays.

기타 정보

For additional information on our range of Biochemicals, please complete this form.

신호어

Danger

유해 및 위험 성명서

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Flam. Liq. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

표적 기관

Central nervous system

Storage Class Code

3 - Flammable liquids


시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

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