P8340

Protease Inhibitor Cocktail

DMSO solution, for the inhibition of serine, cysteine, acid proteases and aminopeptidases, for use with mammalian cell and tissue extracts, DMSO solution

• 동의어(들): Protease Inhibitor Mix
• MDL number: MFCD00677817
• UNSPSC 코드: 12352200
• NACRES: NA.77
• solubility: water: soluble

속성

• 제품명: Protease Inhibitor Cocktail, for use with mammalian cell and tissue extracts, DMSO solution
• 생물학적 소스: bovine lung
• Quality Level: 300
• 양식: DMSO solution
• solubility: water: soluble
• 저장 온도: −20°C

설명

일반 설명

Protease Inhibitor Cocktail for mammalian cell and tissue extracts, in DMSO solution. It contains six individual components for inhibiting serine, cysteine, and acid proteases, and aminopeptidases.

특이성

Inhibits serine, cysteine, and acid proteases, and aminopeptidases.

애플리케이션

Ideal for use as a buffer component during sonication, lysis buffer, and RIPA buffer

Provides protection against endogenous enzyme activity in cell and tissue extracts

생화학적/생리학적 작용

This mixture contains individual components, including AEBSF at 104 mM, Aprotinin at 80 μM, Bestatin at 4 mM, E-64 at 1.4 mM, Leupeptin at 2 mM and Pepstatin A at 1.5 mM. Each component has specific inhibitory properties. AEBSF and Aprotinin act to inhibit serine proteases, including trypsin, chymotrypsin, and plasmin amongst others. Bestatin inhibits aminpeptidases. E-64 acts against cystein proteases. Leupeptin acts against both serine and cystein proteases. Pepstatin A inhibits acid proteases.

특징 및 장점

Broad-spectrum inhibition: inhibits multiple classes of proteases and aminopeptidases

Stable storage: supplied as a solution in DMSO, allowing for long-term storage at -20°C

Consistent performance: ready-to-use solution

성분

AEBSF
Aprotinin
Bestatin
E-64
Leupeptin
Pepstatin A

주의사항

This cocktail should be stored at -20 °C.

제조 메모

One mL of this cocktail solution is recommended for the inhibition of endogenous enzymes found in 100 mL of lysate from 20 g of bovine liver or 10 mL of lysate from CHO cells. This product is supplied as a solution in DMSO.

기타 정보

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

안전성 정보

• 픽토그램: GHS07
• 신호어: Warning
• 유해 및 위험 성명서: H315,H319
• 예방조치 성명서: P264 - P280 - P302 + P352 - P305 + P351 + P338 - P332 + P313 - P337 + P313
• Hazard Classifications: Eye Irrit. 2 - Skin Irrit. 2
• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point (°F): 188.6 °F - closed cup
• Flash Point (°C): 87 °C - closed cup