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Merck
모든 사진(2)

주요 문서

P9290

Sigma-Aldrich

Proteinase K–Agarose from Tritirachium album

lyophilized powder

동의어(들):

Proteinase K-Agarose

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About This Item

MDL number:
UNSPSC 코드:
41106500
NACRES:
NA.56

생물학적 소스

microbial (T.album
T. ALBUM)

Quality Level

양식

lyophilized powder

라벨링 범위

30-140 units per g solid

기술

DNA extraction: suitable
RNA extraction: suitable
protein extraction: suitable

팽창

1 g swells to ~5 mL

저장 온도

−20°C

애플리케이션

Proteinase K-agarose is used in protein and nucleic acid isolation, modifying enzymes and immobilized enzymes. Proteinase K-agarose has been used to study Klebsiella pneumonia as well as Echinococcus multilocularis infection in domesticated dogs.

생화학적/생리학적 작용

The application of proteinase K has been extended by immobilizing it on an insoluble matrix such as agarose. The agarose is quite inert in most systems and allows high activities per unit weight. This reactive resin allows the enzyme reaction to be quickly catalyzed by momentary contact with the substrate in a suitable medium. Reactions can be carried out in a batch slurry or even through a small column. In either case, the insoluble enzyme does not contaminate the reaction mix, which can be readily filtered out of the batch slurry or remain behind in the column.

특징 및 장점

• Recylable Proteinase K on matrix support.
• Spin column or batch processing possible.

단위 정의

One unit will hydrolyze urea-denatured hemoglobin to produce color equivalent to 1.0 μmole (181 μg) of tyrosine per min at pH 7.5 at 37 °C (color by Folin-Ciocalteu reagent).

물리적 형태

Lyophilized powder stabilized with lactose

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


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