추천 제품
생물학적 소스
microbial (T.album
T. ALBUM)
Quality Level
양식
lyophilized powder
라벨링 범위
30-140 units per g solid
기술
DNA extraction: suitable
RNA extraction: suitable
protein extraction: suitable
팽창
1 g swells to ~5 mL
저장 온도
−20°C
애플리케이션
Proteinase K-agarose is used in protein and nucleic acid isolation, modifying enzymes and immobilized enzymes. Proteinase K-agarose has been used to study Klebsiella pneumonia as well as Echinococcus multilocularis infection in domesticated dogs.
생화학적/생리학적 작용
The application of proteinase K has been extended by immobilizing it on an insoluble matrix such as agarose. The agarose is quite inert in most systems and allows high activities per unit weight. This reactive resin allows the enzyme reaction to be quickly catalyzed by momentary contact with the substrate in a suitable medium. Reactions can be carried out in a batch slurry or even through a small column. In either case, the insoluble enzyme does not contaminate the reaction mix, which can be readily filtered out of the batch slurry or remain behind in the column.
특징 및 장점
• Recylable Proteinase K on matrix support.
• Spin column or batch processing possible.
• Spin column or batch processing possible.
단위 정의
One unit will hydrolyze urea-denatured hemoglobin to produce color equivalent to 1.0 μmole (181 μg) of tyrosine per min at pH 7.5 at 37 °C (color by Folin-Ciocalteu reagent).
물리적 형태
Lyophilized powder stabilized with lactose
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
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Lot/Batch Number
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