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SRE0005

Sigma-Aldrich

Proteinase K from Tritirachium album

Reagents designed and manufactured under current ISO 13485 certification.

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Synonym(s):
Endopeptidase K
CAS Number:
Enzyme Commission number:
EC Number:
MDL number:
NACRES:
NA.32

grade

for molecular biology

Quality Level

form

buffered aqueous glycerol solution

mol wt

28.93 kDa

concentration

≥10 mg/mL protein
≥800 unit/mL

impurities

≤500 pg/mg DNA (PicoGreen® assay)

application(s)

diagnostic assay manufacturing

foreign activity

DNAse, RNAse, exonuclease, endonuclease, and nickase, none detected

shipped in

wet ice

storage temp.

2-8°C

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P2308P6556P5568
Quality Level

500

Quality Level

400

Quality Level

300

Quality Level

200

foreign activity

DNAse, RNAse, exonuclease, endonuclease, and nickase, none detected

foreign activity

DNAse, Nickase and RNAse, none detected

foreign activity

Dnase ≤30 Kunitz units/mg solid, RNase ≤0.003 Kunitz units/mg solid

foreign activity

-

impurities

≤500 pg/mg DNA (PicoGreen® assay)

impurities

≤1 ppm DNA

impurities

-

impurities

-

mol wt

28.93 kDa

mol wt

28.93 kDa

mol wt

28.93 kDa

mol wt

28.93 kDa

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

shipped in

-

Application

Useful for the proteolytic inactivation of nucleases during the isolation of DNA and RNA.
Removes endotoxins that bind to cationic proteins such as lysozyme and ribonuclease A.
Reported useful for the isolation of hepatic, yeast, and mung bean mitochondria
Determination of enzyme localization on membranes
Treatment of paraffin embedded tissue sections to expose antigen binding sites for antibody labeling.
Digestion of proteins from brain tissue samples for prions in Transmissible Spongiform Encephalopathies (TSE) research.

Biochem/physiol Actions

Proteinase K is a stable and highly reactive serine protease. Evidence from crystal and molecular structure studies indicates the enzyme belongs to the subtilisin family with an active-site catalytic triad (Asp39-His69-Ser224). It is stable in a broad range of environments: pH, buffer salts, detergents (SDS), and temperature. In the presence of 0.1-0.5% SDS, proteinase K retains activity and will digest a variety of proteins and nucleases in DNA preparations without compromising the integrity of the isolated DNA.

Unit Definition

One unit will hydrolyze urea-denatured hemoglobin to produce color equivalent to 1.0 μmole of tyrosine per min at pH 7.5 at 37 °C (color by Folin-Ciocalteu reagent).

Legal Information

PicoGreen is a registered trademark of Life Technologies

Pictograms

Exclamation markHealth hazard

signalword

Danger

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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Articles

Proteinase K is commonly used in molecular biology and biochemistry applications to digest structural proteins and enzymes. It is useful in removing nucleases that can degrade DNA and RNA, as well as in the isolation of intact genomic DNA from various sources.

The use of Pro K in combination with other reagents, such as detergents and chaotropic agents, can help to disrupt the cell membranes and release DNA from tissue. This is particularly important for downstream applications such as PCR, sequencing, and other molecular biology techniques that require pure and intact DNA.

The cost of proteinase K can vary depending on the source, purity, manufacturing process and vendor’s quality management system. It is important to balance the cost with the desired quality, performance, documentation and technical/quality support to select the optimal Proteinase K for the intended application

In blood DNA extraction, Proteinase K, an enzyme commonly used to degrade proteins, can help break down the cellular and nuclear membranes, releasing DNA from the cells that protect it from degradation and increase purity/yield making it more suitable for various molecular biology techniques.

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