P4850
Proteinase K from Tritirachium album
buffered aqueous glycerol solution, for molecular biology, ≥800 units/mL
Recommended Products
grade
for molecular biology
Quality Level
form
buffered aqueous glycerol solution
mol wt
28.93 kDa
concentration
≥10 mg/mL
≥800 units/mL
impurities
≤0.5 ppm DNA (PicoGreen® assay)
foreign activity
DNase, Nickase and RNase, none detected
storage temp.
2-8°C
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Application
- in bovine endometrial epithelial cells for herpes viral DNA extraction
- for viral RNA extraction from nasal swabs
- as a component of phase lock and direct PCR lysis buffer
Removes endotoxins that bind to cationic proteins such as lysozyme and ribonuclease A.
Reported useful for the isolation of hepatic, yeast, and mung bean mitochondria
Determination of enzyme localization on membranes
Treatment of paraffin embedded tissue sections to expose antigen binding sites for antibody labeling.
Digestion of proteins from brain tissue samples for prions in Transmissible Spongiform Encephalopathies (TSE) research.
Biochem/physiol Actions
Unit Definition
Physical form
Legal Information
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
wgk_germany
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter
Certificates of Analysis (COA)
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Articles
Proteinase K aids in molecular biology applications by digesting structural proteins, removing nucleases, and isolating intact genomic DNA.
Balancing Proteinase K cost with quality and technical support ensures optimal enzyme selection for diverse applications.
Pro K aids in disrupting cell membranes for DNA release, crucial for downstream molecular biology techniques.
In blood DNA extraction, Proteinase K, an enzyme commonly used to degrade proteins, can help break down the cellular and nuclear membranes, releasing DNA from the cells that protect it from degradation and increase purity/yield making it more suitable for various molecular biology techniques.
Protocols
Proteinase K activity measured via spectrophotometry using hemoglobin substrate, crucial for enzyme characterization.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
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