539480
Proteinase K from Tritirachium album
Synonym(s):
Endopeptidase K
About This Item
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form
lyophilized solid
Quality Level
specific activity
≥30 mAnson units/mg dry wt
≥40 mAnson units/mg protein
mol wt
28.93 kDa
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
color
white
solubility
50 mM Tris-HCl, 2 mM calcium acetate, pH 8.0: soluble
foreign activity
DNase, none detected (nicking activity with pBR322, incubation for 6 h at 37°C)
RNase, none detected (ribonuclease activity with MS2 RNA detected after incubation for 6 h at 37°C)
shipped in
ambient
storage temp.
2-8°C
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General description
Application
Removes endotoxins that bind to cationic proteins such as lysozyme and ribonuclease A.
Reported useful for the isolation of hepatic, yeast, and mung bean mitochondria
Determination of enzyme localization on membranes
Treatment of paraffin embedded tissue sections to expose antigen binding sites for antibody labeling.
Digestion of proteins from brain tissue samples for prions in Transmissible Spongiform Encephalopathies (TSE) research.
Biochem/physiol Actions
Warning
Unit Definition
Physical form
Reconstitution
Other Notes
Lebherz, H.G., et al. 1986. Biochem. J.233, 51.
Ebeling, W., et al. 1974. Eur. J. Biochem.47, 91.
Legal Information
signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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