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  • Secondary Structure of Influenza A Virus Genomic Segment 8 RNA Folded in a Cellular Environment.

Secondary Structure of Influenza A Virus Genomic Segment 8 RNA Folded in a Cellular Environment.

International journal of molecular sciences (2022-03-11)
Barbara Szutkowska, Klaudia Wieczorek, Ryszard Kierzek, Pawel Zmora, Jake M Peterson, Walter N Moss, David H Mathews, Elzbieta Kierzek
초록

Influenza A virus (IAV) is a member of the single-stranded RNA (ssRNA) family of viruses. The most recent global pandemic caused by the SARS-CoV-2 virus has shown the major threat that RNA viruses can pose to humanity. In comparison, influenza has an even higher pandemic potential as a result of its high rate of mutations within its relatively short (<13 kbp) genome, as well as its capability to undergo genetic reassortment. In light of this threat, and the fact that RNA structure is connected to a broad range of known biological functions, deeper investigation of viral RNA (vRNA) structures is of high interest. Here, for the first time, we propose a secondary structure for segment 8 vRNA (vRNA8) of A/California/04/2009 (H1N1) formed in the presence of cellular and viral components. This structure shows similarities with prior in vitro experiments. Additionally, we determined the location of several well-defined, conserved structural motifs of vRNA8 within IAV strains with possible functionality. These RNA motifs appear to fold independently of regional nucleoprotein (NP)-binding affinity, but a low or uneven distribution of NP in each motif region is noted. This research also highlights several accessible sites for oligonucleotide tools and small molecules in vRNA8 in a cellular environment that might be a target for influenza A virus inhibition on the RNA level.

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제품 설명

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Monoclonal Anti-Influenza A virus H1N1 HA (Hemagglutinin) antibody produced in mouse, clone GT223, affinity isolated antibody
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Goat Anti-Mouse IgG Antibody, HRP conjugate, Upstate®, from goat
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Rabbit Anti-Mouse IgG Antibody, FITC conjugate, Chemicon®, from rabbit
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Anti-Influenza A Antibody, nucleoprotein, clone A1, clone A1, Chemicon®, from mouse
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Goat Anti-Rabbit IgG Antibody, (H+L) HRP conjugate, 1 mg/mL, Chemicon®