Antagonistic and synergistic effects of light irradiation on the effects of copper on Chlamydomonas reinhardtii.

Antagonistic and synergistic effects of light irradiation on the effects of copper on Chlamydomonas reinhardtii.

Aquatic toxicology (Amsterdam, Netherlands) (2014-07-30)

Giulia Cheloni, Claudia Cosio, Vera I Slaveykova

초록

The present study showed the important role of light intensity and spectral composition on Cu uptake and effects on green alga Chlamydomonas reinhardtii. High-intenisty light (HL) increased cellular Cu concentrations, but mitigated the Cu-induced decrease in chlorophyll fluorescence, oxidative stress and lipid peroxidation at high Cu concentrations, indicating that Cu and HL interact in an antagonistic manner. HL up-regulated the transcription of genes involved in the antioxidant response in C. reinhardtii and thus reduced the oxidative stress upon exposure to Cu and HL. Combined exposure to Cu and UVBR resulted in an increase of cellular Cu contents and caused severe oxidative damage to the cells. The observed effects were higher than the sum of the effects corresponding to exposure to UVBR or Cu alone suggesting a synergistic interaction.

MATERIALS

제품 번호

브랜드

제품 설명

254177

Sigma-Aldrich

Copper, beads, 2-8 mm, 99.9995% trace metals basis

266744

Sigma-Aldrich

Copper, foil, thickness 1.0 mm, 99.999% trace metals basis

326429

Sigma-Aldrich

Copper, wire, diam. 0.25 mm, 99.999% trace metals basis

349151

Sigma-Aldrich

Copper, foil, thickness 0.5 mm, 99.98% trace metals basis

326410

Sigma-Aldrich

Copper, wire, diam. 0.64 mm, 99.995% trace metals basis

349216

Sigma-Aldrich

Copper, wire, diam. 1.0 mm, ≥99.9%

349208

Sigma-Aldrich

Copper, foil, thickness 0.025 mm, 99.98% trace metals basis

326488

Sigma-Aldrich

Copper, beads, 2-8 mm, ≥99.99% trace metals basis

BCR058

Continuous cast copper (O), BCR^®, certified reference material, rod

GF05237209

Copper, tube, 500mm, outside diameter 0.25mm, inside diameter 0.05mm, wall thickness 0.1mm, as drawn, 99.9%

GF20205368

Copper - O.F.H.C., foil, 10mm disks, thickness 0.7mm, half hard, 99.95+%

GF19723136

Copper - O.F.H.C., foil, 25mm disks, thickness 0.125mm, hard, 99.95+%

GF32166761

Copper - O.F.H.C., foil, 5m coil, thickness 0.1mm, coil width 100mm, hard, 99.95+%

GF81712098

Copper, tube, 200mm, outside diameter 3.0mm, inside diameter 1.2mm, wall thickness 0.9mm, as drawn, 99.9%

GF82329172

Copper, tube, 200mm, outside diameter 0.95mm, inside diameter 0.29mm, wall thickness 0.33mm, as drawn, 99.9%

GF03625051

Copper - O.F.H.C., foil, 25mm disks, thickness 0.075mm, half hard, 99.95+%

GF08771766

Copper - O.F.H.C., foil, 1m coil, thickness 0.1mm, half hard, 99.95+%

GF03331268

Copper, tube, 500mm, outside diameter 2.5mm, inside diameter 1.9mm, wall thickness 0.3mm, as drawn, 99.9%

GF14011647

Copper, tube, 500mm, outside diameter 3.0mm, inside diameter 1.2mm, wall thickness 0.9mm, as drawn, 99.9%

GF99677165

Copper, wire reel, 1000m, diameter 0.05mm, annealed, 99.9%

GF37244729

Copper, tube, 100mm, outside diameter 3.5mm, inside diameter 1.56mm, wall thickness 0.97mm, as drawn, 99.9%

GF10425169

Copper - O.F.H.C., foil, 20m coil, thickness 0.010mm, coil width 25mm, hard, 99.95+%

GF87158650

Copper, tube, 500mm, outside diameter 1.0mm, inside diameter 0.24mm, wall thickness 0.38mm, as drawn, 99.9%

GF53132330

Copper - O.F.H.C., foil, 50mm disks, thickness 0.075mm, half hard, 99.95+%

GF93055901

Copper - O.F.H.C., foil, 2m coil, thickness 0.025mm, hard, 99.95+%

GF09290426

Copper - O.F.H.C., foil, 300x300mm, thickness, 0.5mm, half hard, flattened, 99.95+%

GF08494577

Copper, wire reel, 1000m, diameter 0.075mm, annealed, 99.9%

GF58516285

Copper - O.F.H.C., foil, 20m coil, thickness 0.05mm, coil width 10mm, annealed, 99.95+%

GF93468325

Copper, wire reel, 0.2m, diameter 0.1mm, aluminum cladding, outside diameter 0.25mm, 99.9%

GF65221512

Copper - O.F.H.C., foil, 5m coil, thickness 0.05mm, coil width 300mm, half hard, 99.95+%