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Merck
  • Novel high-throughput and widely-targeted liquid chromatography-time of flight mass spectrometry method for d-amino acids in foods.

Novel high-throughput and widely-targeted liquid chromatography-time of flight mass spectrometry method for d-amino acids in foods.

Journal of bioscience and bioengineering (2016-09-21)
Yutaka Konya, Moyu Taniguchi, Eiichiro Fukusaki
초록

Recently, the demand for d-amino acid profiling has been drastically increasing because the significance of d-amino acid in various biological events is suggested. However, the present methodologies for d-amino acid profiling are still unsatisfactory. Therefore, a highly sensitive, robust, high-throughput, and user-friendly method for d-amino acid profiling must be developed. In this paper, we developed a novel method for d-amino acid profiling using a combination of a chiral column and time of flight mass spectrometry (TOFMS). To our knowledge, our approach has the best performance for d-amino acid analysis that includes the shortest analytical time (within 10 min), the highest enantioseparability without derivatization, and the largest coverage for analytical targets (more than one hundred targets including non-proteinogenic amino acids and amines). Thus, our novel profiling method will be instrumental in advancing the d-amino acid research in the future.

MATERIALS
제품 번호
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Sigma-Aldrich
DL-Kynurenine, ≥95.0% (NT)
Sigma-Aldrich
DL-Alanine-2,3,3,3-d4, 98 atom % D
Sigma-Aldrich
DL-Lysine monohydrochloride, ≥98% (HPLC)
Sigma-Aldrich
Hypotaurine, ≥98% (TLC)
Sigma-Aldrich
DL-Ethionine, ≥95% (TLC)
Sigma-Aldrich
Cystathionine, ≥90% (HPLC)