D3937

DirectLoad^™ 1 kb DNA Ladder

ready-to-use marker for DNA electrophoresis

• Synonym(s): 1 kb marker, 1kb gel marker, DNA marker, agarose gel electrophoresis marker
• NACRES: NA.25
• UNSPSC Code: 41105335

คุณสมบัติ

• Quality Level: 200
• form: liquid
• usage: 100 uses
• suitability: suitable for electrophoresis (DNA)
• storage temp.: −20°C

คำอธิบาย

General description

Sigma′s DirectLoad^™ 1 kb Ladder contains 11 fragments consisting of 500 bp repeats from 0.5 to 3 kb, 1 kb repeats from 3 to 6 kb, and 2 kb repeats from 6 to 10 kb. For NA electrophoresis, 5 ul of the marker can be loaded directly into a single lane on an agarose or polyacrylamide gel. Suitable for use in Northern and Southern blotting.

Application

Suitable for size determination of dsDNA by DNA electrophoresis with either agarose or polyacrylamide gels.

Features and Benefits

• Ready-to-load
• Easy-to-use
• Popular band sizes

Other Notes

Contains 11 fragments consisting of 500 bp repeats from 0.5 to 3 kb, 1 kb repeats from 3 to 6 kb and 2 kb repeats from 6 to 10 kb.

For optimal resolution, the recommended agarose gel concentration is 0.75%.

Sigma′s DirectLoad^™ 1kb Ladder is provided in a gel-loading solution of 2.5% Ficoll (Type 400), 0.0125% bromophenol blue, and 0.00625% xylene cyanol.

Legal Information

DirectLoad is a trademark of Sigma-Aldrich Co. LLC

ข้อมูลความปลอดภัย

• คลาสการจัดเก็บ: 12 - Non Combustible Liquids
• wgk: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable