HomeWebinarsHow to Increase The Cost Effectiveness for HPLC Separations of Challenging and Matrix-rich Samples

How to Increase The Cost Effectiveness for HPLC Separations of Challenging and Matrix-rich Samples


Matrix-rich samples often require extensive sample preparation when analyzed with HPLC and UHPLC. This is a substantial time and cost factor. A robust column material enabling a simplified sample preparation will save time and improve the lab productivity.

The performance of HPLC columns has improved dramatically in recent years, particularly in terms of separation power as measured by the number of theoretical plates per meter. The improvement in performance has been achieved primarily by a reduction in particle size.

Not all samples have benefited equally from this development: Clean samples with a relatively simple matrix are ideally suited for this new generation of columns. But for samples with a more difficult and complex matrix, the benefits are often accompanied by significant limitations. The explanation is quite simple; the smaller the particle size and the higher the sample amount injected, the more easily a column plugs or blocks. If the sample contains a matrix with colloidal and micro-particulate matter in suspension, then it becomes necessary to perform a rigorous sample “clean-up” before injection. This demands additional time for sample preparation steps, and of course involves additional cost.
The potential for cost and time savings by increasing lab productivity with faster separations and column materials providing very high matrix tolerance will be discussed.

By attending this presentation you will...

  • Understand the challenges for matrix-rich sample analysis using HPLC and/or UHPLC and how to overcome them
  • Learn how to leverage lab efficiency by simplifying the HPLC workflow using monolithic silica columns.


Petra Lewits

Petra Lewits


Global Product Manager for Analytical Chromatography, HPLC columns

Since 2002, Petra Lewits has been the Global Product Manager for Analytical Chromatography at MilliporeSigma. She has more than 30 years of experience in HPLC, including method development and validation, sales, and marketing.

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