ISO 11133:2014 is a mandatory standard for all ISO 17025-accredited labs for microbiological analysis of food, feed and water. It describes the preparation, production and performance testing of culture media from this whole area. Recently published amendment 1:2018 contains corrections and additional explanations whilst future amendment 2 will describe the performance testing of confirmation media and reagents.
ISO 16649-1, which specifies a horizontal method for the enumeration of β-glucuronidase-positive Escherichia coli, has also been revised. The method uses a colony-count technique after resuscitation using membranes and incubation at 44 °C on TBX agar.
There are three horizontal methods (ISO 16649-1, ISO 16649-2 and ISO 16649-3) for the enumeration of β-glucuronidase-positive Escherichia coli, all parts are for general application. Parts 1 and 3 include a resuscitation step and should be used in preference for foodstuffs likely to contain sub-lethally injured cells.
This webinar explains the latest changes in ISO 11133 and ISO 16649 and will give an outlook on the future of both standards.
Senior Scientist Traditional Microbiology
Barbara has over 30 years of industrial microbiology experience and is currently at MilliporeSigma in Regulatory Marketing. She is an active member in several national and international bodies addressing microbiological topics in ISO, CEN and IFU, including the ISO Working Groups ‘Culture media’ and ‘Quality control of membrane filters’, and was the responsible project leader for the new EN ISO 7704:2023.
Andreas Bubert, PhD
Senior Product Manager, Culture Media Food & Beverage
Andreas received a PhD in molecular microbiology for his research and diagnostics work on Listeria. He has over 24 years’ experience at MilliporeSigma, focusing on microbiological solutions for the food and beverage industries. He is a member of AOAC RI Advisory Council and has authored over 50 publications.
Food and beverage testing and manufacturing
Session 1:presented November 19, 2018
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