HomeWebinarsUse HPTLC to Get Insights into Sample Composition

Use HPTLC to Get Insights into Sample Composition


What Does It Cover?

Thin-Layer Chromatography has been a well-known method for analysis of botanicals and other complex samples since the end of the 1930s. Today’s modern thin-layer chromatography combines the advantage of analytical robustness and high sample throughput with the possibility to use all kind of specific detection methods, e.g. classical UV/Vis/Fluorescence detection, mass spectrometry or effect-directed analysis. This method increases the amount of information for a fast and efficient screening for new compounds and the identification of raw materials especially for samples with a high matrix load such as herbal drugs, cosmetic and food samples.

HPTLC, the most advanced form of Thin-Layer Chromatography, is a powerful yet simple and cost effective tool for testing identity, purity, and strength (content) of botanicals as well as excluding adulteration during quality control. With the publication of general chapters by the United States Pharmacopoeia (USP <203>) and European Pharmacopoeia (Ph.Eur. 2.8.25) HPTLC has officially come into existence as a highly standardized and therefore reproducible analytical technique. The use of high performance plates, suitable instrument and software, a standardized methodology, and validated methods ensures reliable results that are fully compliant with current Good Manufacturing Practice (cGMP). HPTLC fingerprints allow convenient visual comparison of multiple samples even if those originate from different plates (and different laboratories worldwide). Reference images (HPTLC fingerprints of botanical reference materials or other references) can be used to qualify data and pass/fail samples based on similarity or difference.

We will give you an overview about the versatility of HPTLC, applicable to many of your analytical tasks. Don’t miss the chance to extend your knowledge!

This webinar will focus on:

  • HPTLC for quality control of botanicals, food, and other complex samples
  • Using HPTLC for lead substance identification in combination with on-plate activity screening
  • Structure elucidation using direct HPTLC/MS-coupling

What Will You Learn?

  • To perform standardized HPTLC
  • To use HPTLC in quality control
  • To get multiple information from one separation (by multiple detection)
  • To use HPTLC for lead substance identification


Melanie Broszat, Ph.D.

Melanie Broszat, Ph.D.

CAMAG, Muttenz, Switzerland

Scientific Business Development Manager

Dr. Melanie Broszat has been working at CAMAG as Scientific Business Development Manager since April 2014. In this position, she is responsible for the scientific evaluation of the different HPTLC markets, gives trainings and seminars, and builds-up an HPTLC-expert-network. Dr. Melanie Broszat did her Ph.D. in molecular biology and environmental microbiology under the supervision of Prof. Dr. Elisabeth Grohmann at the Albert-Ludwigs-University in Freiburg, Germany, from 2010/07-2013/12. Her research interests focused on the development of new analytical tools for detection and identification of microorganisms. From 2010/03-2010/06, she has worked in Research as Laboratory engineer in the University of Applied Sciences in Offenburg, Germany. From 2009/09-2010/02, she has worked in QA of a Food company and from 2008/08-2009/08 in Research as Laboratory engineer on the development of HPTLC methods in the food and environmental application fields together with Prof. Dr. Bernd Spangenberg in the University of Applied Sciences Offenburg, Germany. From 2004/10-2008/08 she has studied Process Engineering at the University of Applied Sciences in Offenburg, Germany.

Michaela Oberle

Michaela Oberle


R&D Project leader Thin Layer Chromatography, Instrumental analytics

Michaela Oberle started her career at MilliporeSigma as a lab technician apprentice in 1992. She took over responsibilities in various departments and became specialist for analytical, preparative and flash chromatography. In 2000 she joined the Pigments and Cosmetics Research team in Darmstadt, Germany and held an R&D specialist position, where she was responsible for the extraction and analysis of natural compounds as well as for cosmetic efficacy screenings including skin and hair applications. Since 2012 Michaela Oberle acts as a R&D specialist in the Advanced analytics “Thin layer chromatography” department and took over responsibility for product development, customer requests, TLC applications and international trainings for TLC issues. She holds a degree of Bachelor of science in Business Chemistry.

Webinar Information

Analytical chemistry

  • Mass spectrometry
  • Duration:56min

  • Language:English

  • Session 1:presented February 20, 2018

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