This seminar will focus on strategies and techniques to maximize efficiency and resolution when using reversed phase chromatography (RPC) and size exclusion chromatography (SEC) in analyzing biomacromolecules. Aspects of method development and troubleshooting will be examined for both modes of chromatography.
At the conclusion of the seminar, the engaged attendee will learn:
Analyzing biomacromolecules is a unique analytical challenge owing to a number of product-related impurities and the complexity of the analytes. This complexity is a result of the myriad number of modifications to these analytes in each sample including, but not limited to, phosphorylation, methylation, and glycosylation. Due to the heterogeneity of an individual analyte, in addition to a biomolecule’s tendency to interact with silica-based, reversed-phase stationary phases, the resulting chromatographic peak can exhibit extensive broadening and tailing. To further complicate matters, most large proteins do not ionize well using conventional electrospray ionization mass spectrometers. In addition, when a mixture of proteins is not well-resolved, chromatographically, prior to the mass spectrometer, further ion suppression can occur. At a higher level, sample heterogeneity also increases with aggregated and fragmented proteins. This is routinely determined by size exclusion chromatography, often lacking a deeper characterization of the impurities. Therefore, the need for highly efficient, robust, and reproducible analytical methods is not a suggestion, but a requirement, for accurate characterization of proteinaceous samples.
Tosoh Bioscience GmbH
Product Manager, Analytical Columns
Andrea Krumm studied biotechnology before gaining a PhD in Cell and Cancer Biology. After four years as an applications specialist for optical analytical devices used in academic and pharmaceutical research, she joined Tosoh Bioscience GmbH in 2020 as a product manager for analytical columns. Andrea is responsible for gathering customer requirements, application areas and addressing these with new and existing products of the analytical columns line.
Cory E. Muraco
Biomolecule Workflows Manager
Cory started his career at MilliporeSigma in 2013, joining the Chemical Standards R&D group where he was responsible for developing LC and GC methods for assaying the purity of Certified Reference Materials (CRMs). In 2015, Cory joined the HPLC R&D group where he developed applications around biomolecule analysis using multiple modes of chromatography and detection techniques. In 2019, Cory joined the marketing organization as the Global Franchise Product Manager for HPLC columns. In 2022, Cory assumed his current role as the Biomolecule Workflows Manager designing, developing, and executing the marketing and R&D strategies around the bioanalytical initiative. An author of several manuscripts appearing in trade magazines and has delivered over 100 presentations at international conferences, round table symposia, and at various pharmaceutical and biopharmaceutical companies.
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