MilliporeSigma

POLR2A

polymerase (RNA) II (DNA directed) polypeptide A, 220kDa

Synonyms:
POLR2, POLRA, RPB1, RPBh1, RPO2, RPOL2, RpIILS, hRPB220, hsRPB1
Species:
UniProtKB ID:
Gene ID:
  • Human(5430) Summary: This gene encodes the largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, this subunit, in combination with several other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA. [provided by RefSeq, Jul 2008]
  • Mouse(20020) polymerase (RNA) II (DNA directed) polypeptide A
  • Rat(363633) polymerase (RNA) II (DNA directed) polypeptide A
  • cow(282312) polymerase (RNA) II (DNA directed) polypeptide A, 220kDa
  • naked mole-rat(101725588) polymerase (RNA) II (DNA directed) polypeptide A, 220kDa
  • dog(479483) polymerase (RNA) II (DNA directed) polypeptide A, 220kDa
  • Zebrafish(553347) polymerase (RNA) II (DNA directed) polypeptide A
  • sheep(100144762) polymerase (RNA) II (DNA directed) polypeptide A, 220kDa
  • domestic cat(101098500) polymerase (RNA) II (DNA directed) polypeptide A, 220kDa
  • domestic guinea pig(100734232) polymerase (RNA) II (DNA directed) polypeptide A, 220kDa

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Antibodies

Product Number
Description
Species Reactivity
Application
Anti-Phospho-POLR2A-S7 antibody produced in rabbit, ,
Species Reactivity
human, mouse, rat
Application
western blot
Anti-Phospho-POLR2A-S5 antibody produced in rabbit, ,
Species Reactivity
human, mouse, rat
Application
immunofluorescence
immunohistochemistry

western blot
Anti-POLR2A Antibody, clone 8H4A2, Rabbit Monoclonal, ,
Species Reactivity
mouse
Application
western blot
Anti-phospho RNA Pol II (Ser7), clone 3D4A12 Antibody, clone 3D4A12, 1 mg/mL, from rat,
Species Reactivity
canine, human, monkey, mouse, rat
Application
ChIP
ELISA

immunocytochemistry

western blot
Anti-phospho-RNAPII Antibody (Thr4), clone 1G7, clone 1G7, from rat,
Species Reactivity
human, mouse, yeast
Application
ChIP
immunocytochemistry

immunoprecipitation (IP)

western blot

Proteins

Product Number
Description
Species
RNA Polymerase II, C-terminal domain, GST tagged human, recombinant, expressed in E. coli, ≥70% (SDS-PAGE),
Species
human
RNA Polymerase II, C-terminal human, recombinant, expressed in E. coli, ≥85% (SDS-PAGE),
Species
human

esiRNA

Product Number
Description
Species
MISSION® esiRNA, targeting human POLR2A,
Species
human
MISSION® esiRNA, targeting mouse Polr2a,
Species
mouse