93711
Atto 655
BioReagent, suitable for fluorescence, ≥85% (HPLC)
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product line
BioReagent
Quality Level
assay
≥85% (HPLC)
form
powder
manufacturer/tradename
ATTO-TEC GmbH
transmittance
254 nm
655 nm
fluorescence
λex 655 nm; λem 680 nm in 0.1 M phosphate pH 7.0
λ
in ethanol (with 0.1% trifluoroacetic acid)
UV absorption
λ: 652-658 nm Amax
suitability
suitable for fluorescence
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Application
Atto labels are designed for highest sensitivity applications. A unique combination of advantages makes them highly favorable tools for all kinds of labeling applications. Some of their properties make them specifically interesting for single molecule detection. Atto labels are based on rigid structures and do not show any cis-trans-isomerization, which lowers the brightness of signals and leads to environment dependency, e.g., spectral shifts by conjugation.
Atto 655 shows a molar extinction of 110,000 and QY of 30% in water (50% in ethanol). Decay time is 1.9 ns.
Atto 655 shows a molar extinction of 110,000 and QY of 30% in water (50% in ethanol). Decay time is 1.9 ns.
Other Notes
New red absorbing fluorescent dye with best signal-to-noise ratio and long fluorescence life-time. Useful as a biophysical probe for binding interactions.[1]
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Articles
Fluorescence lifetime measurement is advantageous over intensity-based measurements. Applications include fluorescence lifetime assays, sensing and FLI.
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