MilliporeSigma

Tryptic Soy Broth

Synonym: Casein Soya Broth (CASO), Soybean Casein digest Broth, TSB, Tryptone Soya Broth, Soybean Casein Digest Medium (SCDM)

Tryptic Soy Broth bottle

Tryptic Soy Broth, commonly referred to as Tryptone soya broth or soybean casein digest broth, is a liquid medium utilized in microbiology. Its primary purposes include detecting sterility, cultivating fastidious microorganisms, and enriching them. The broth comprises enzymatic digests of soybean meal and casein, peptones that provide amino acids and nitrogenous compounds. Additionally, it contains sodium chloride to maintain osmotic equilibrium, and dextrose, which provides carbohydrates and energy. This medium also includes vital vitamins and minerals, which are important for the growth of organisms. Dipotassium hydrogen phosphate is included as a buffering agent to maintain the pH balance. Tryptic Soy Broth is used to culture a wide range of aerobic gram-positive and gram-negative bacteria, fungi, routine blood cultures, and microbial biofilms.


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Tryptic Soy Broth in Pathogen Testing

CASO Broth, also known as casein-peptone soymeal-peptone broth, is a variation of tryptic soy broth. It is utilized to examine the sterility of products or preparations that are not guaranteed to be sterile. They are used to identify specified microorganisms and total viable aerobic counts through the most probable number (MPN) method. These broths function as pre-enrichment media for detecting foodborne pathogens like Salmonella and Shiga toxin-producing E. coli.

Tryptic Soy Broth in Sterility Testing

Tryptic soy broth with neutralizers TLHC (containing tween® 80, lecithin, histidine, and cystine), 2x concentrated tryptic soy broth, or tryptic soy broth with tween® 80 are useful for isolating and cultivating delicate bacteria and yeast.

Tryptic Soy Broth, Vegitone

Vegitone, a tryptic soya broth is a medium in which casein has been substituted with plant peptone. It is commonly used for testing the sterility of molds and less common bacteria. It is free from animal-derived materials. Also, it serves as a non-selective enrichment medium specifically designed for culturing Streptococci. It is the best choice if no animal-derived components are desired in the process.

Tryptic Soy Broth halal

Tryptic soy broth halal is a certified medium used for testing and production where haram products (like pork-derived products) are prohibited.

Ecocult® Tryptic Soy Broth

Ecocult® Tryptic Soy Broth is ideal for the cultivation and isolation of fastidious aerobic bacteria, yeast, and molds. It is also used for sterility testing and microbial enumeration of substances, preparations, and products that lack sterility.

Soybean casein digest broth

Soybean casein digest broth double-packed is utilized as a medium for sterility assessment and helps to identify aerobic bacteria and fungi. The broth plays a significant role in bioburden and sterility testing through membrane filtration or direct inoculation.

Tryptic Soy Broth in Food

GranuCult®, a modified tryptic soy broth (mTSB) is used to enrich E. coli and Salmonella species in red meats. The use of mTSB media effectively inhibits the growth of other gram-negative Enterobacteriaceae species by 90%. GranuCult® with Novobiocin is used for enriching enterohemorrhagic E. coli (EHEC) and detecting E. coli serotype O157:H7 in food samples. The mTSB broth selective supplement contains lyophilized Novobiocin sodium salt, which can effectively suppress the growth of concurrent Gram-positive bacteria during the cultivation of E. coli (O157:H7). GranuCult® is used for media that are granulated for low dust exposure and better dehydration.

Irradiated Tryptic Soy Broth

Tryptic soy broth, non-animal origin, tryptic soy broth irradiated; and tryptic soy (CASO) broth γ-irradiated are useful for simulating aseptic processes in aseptic production lines or conducting media fill tests. These nutrient-rich broths are also ideal for cultivating fastidious microorganisms.

NutriSelect® prime Tryptic Soy Broth

NutriSelect® prime tryptic soy broth, which is halal, is a reliable medium used for growing and culturing a wide range of microorganisms extracted from test samples. It finds application in research as well as in the quality control of food, water, and beverages.



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