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Membrane Immunodetection Devices

A professional in a lab coat and purple gloves working with a Membrane Immunodetection device in a well-equipped laboratory, surrounded by various laboratory equipment and supplies, highlighting the precision and care involved in immunodetection processes.

Western blotting immunodetection involves the use of antibodies to detect target protein. Blotted membranes containing transferred proteins are blocked, followed by antibody incubation and detection using chemiluminescence, colorimetric, or fluorescent methods. Membrane immunodetection devices facilitate the processing of Western blots by simplifying workflows and accelerating detection times. 

Section Overview

The Snap I.D. 2.0 protein detection system, showcasing a sleek white base with two blue adjustment knobs and a viewing screen, alongside designated areas for sample placement, embodying modern protein analysis technology

SNAP i.d.® 2.0 Protein Detection System

The SNAP i.d.® 2.0 Protein Detection System is a unique, vacuum-driven system that pulls reagents through the membrane, increasing membrane contact while eliminating dependence on diffusion or agitation. This improvement in three-dimensional reagent distribution decreases the length of time required for immunodetection. While traditional Western blotting requires 4 to 24 hours for the blocking, antibody incubation, and washing steps, the SNAP i.d.® 2.0 protocol takes only 30 minutes with no loss of signal intensity or reduction in blot quality. All immunodetection steps after protein transfer to a membrane (i.e., blocking, washing, and primary and secondary antibody incubations) can be performed with the SNAP i.d.® 2.0 System.

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An overview of the principles and applications of Western blotting for protein detection. Includes links to detailed protocols, videos, and other resources for protein extraction, gel electrophoresis, transfer to PVDF or nitrocellulose membranes, and chemiluminescent, colorimetric, and fluorescent detection methods.
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